Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Egfl8

Uniprot No.

Q6GUQ1

Research Area

Cell Biology

Alternative Names

Egfl8; Ng3Epidermal growth factor-like protein 8; EGF-like protein 8

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

29-293aa

Target Protein Sequence

GSFKESLGVCSKQTLLVPLRYNESYSQPVYKPYLTLCAGRRICSTYRTTYRVAWREVRREVPQTHVVCCQGWKKPHPGALTCDAICSKPCLNGGVCTGPDRCECAPGWGGKHCHVDVDECRASLTLCSHGCLNTLGSFLCSCPHPLVLGLDGRTCAGGPPESPTSASILSVAVREADSEEERALRWEVAELRGRLEKLEQWATQAGAWVRAVLPMPPEELRPEQVAELWGRGDRIESLSDQVLLLEERLGACACEDNSLGPSLRG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

49.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This recombinant Mouse Egfl8 protein is typically achieved by the manipulation of Egfl8 gene expression in E.coli cells so that it expresses large amounts of a recombinant Egfl8 gene. In order to get enough amount of the Egfl8 protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the Egfl8 protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.

Egfl8 plays negative regulatory roles in mouse thymic epithelial cells (TECs) and thymocytes. It has shown that Egfl8 is downregulated in gastric cancer (GC) and is closely associated with high tumor-node-metastasis stage and dismal prognosis in gastric and colorectal cancer. In addition to participating in thymopoiesis, Egfl8 is also involved in cell migration and invasion by regulating Notch signaling. Egfl8 also can act as a neuritogen and rewire cellular signaling by activating kinases involved in neurogenesis.

Target Background

Gene References into Functions

Mouse EGFL8 encodes a protein of 293 amino acids with two EGFL domains, an Emilin-like domain and a Ca(2+)-binding EGFL domain, which has a molecular mass of 32 kDa. PMID: 26096343
EGFL8 inhibited thymocyte proliferation and induced thymocyte apoptosis. PMID: 23877103
these data show that EGFL8 exerts inhibitory effects on thymic epithelial cells and thymocytes, suggesting that EGFL8 acts as a negative regulatory molecule in the development of T cells in the mouse thymus. PMID: 22828506

Subcellular Location

Secreted.

Tissue Specificity

Ubiquitously expressed in brain, kidney, thymus and lung.

Database Links

KEGG: mmu:81701

STRING: 10090.ENSMUSP00000015611

UniGene: Mm.231343

Code	CSB-EP753592MO
Abbreviation	Recombinant Mouse Egfl8 protein
MSDS	MSDS Datasheet
Size	US$256
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Epidermal growth factor-like protein 8 (Egfl8)

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