Recombinant Mouse Forkhead box protein O4 (Foxo4)

Code CSB-YP894383MO
MSDS
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Source Yeast
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Code CSB-EP894383MO
MSDS
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Source E.coli
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Code CSB-EP894383MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894383MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP894383MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Foxo4; Afx; Afx1; Fkhr3; Mllt7Forkhead box protein O4; Afxh; Fork head domain transcription factor AFX1
Species
Mus musculus (Mouse)
Expression Region
1-505
Target Protein Sequence
MDPENKKSAT GAAAILDLDP DFEPQSRPRS CTWPLPRPDL ATEPHEPSEV EPSLGQKVPT EGHSEPILLP SRLPEPAGGP QPGILGAVTG PRKGGSRRNA WGNQSYAELI SQAIESAPEK RLTLAQIYEW MVRTVPYFKD KGDSNSSAGW KNSIRHNLSL HSKFIKVHNE ATGKSSWWML NPDGGKGGKA PRRRAASMDS SSKLLRGRSK GPKKKPSVLP APPEGATPRS PLGHFAKWSS SPCPRNREEA DVWTTFRPRS SSNASTVSTR LSPMRPESEV LAEEEMPASA SSYAGGVPPT LSEDLELLDG LNLASPHSLL SRSGLSGFSL QHPGLAGPLH SYGASLFGPI DGSLSAGEGC FSSSQSLEAL LTSDTPPPPA DVLMTQVDPI LSQAPTLLLL GGMPSSSKLG TGVSLCPTPL EGPGPSNLVP NLSVMAPPPV MAGAPIPKVL GTPVLASPTE DSSHDRMPQD LDLDMYMENL ECDMDNIISD LMDGEGLDFN FEPDP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Represses smooth muscle cell differentiation by inhibiting the transcriptional coactivator activity of myocardin.
Gene References into Functions
  1. FOXO transcription factors play a salutary role in the protection against the diet-induced fatty liver disease. PMID: 28300161
  2. FoxOs exacerbate the loss of cancellous bone mass associated with type 1 diabetes. PMID: 27491024
  3. Foxo1, Foxo3a, and Foxo4 in chondrocytes regulate endochondral bone formation. PMID: 26232637
  4. The effect of Sirt1 stimulators on osteoclastogenesis was abrogated in cells lacking FoxO1, FoxO3, and FoxO4. PMID: 26287518
  5. FoxO4 is collectively required to maintain MODYrelated gene networks, which in turn are required to enable a gene expression program that permits proper substrate selection (glucose versus fatty acids) for mitochondrial oxidative phosphorylation. PMID: 25264246
  6. FoxO4 transcription factor is present during both oocyte and embryo in vivo maturation and FoxOs may regulate in vitro embryo development under stress conditions. PMID: 25929834
  7. FoxO4 activates Arg1 transcription in endothelial cells in response to MI, leading to downregulation of nitric oxide and upregulation of neutrophil infiltration to the infarct area. PMID: 26438688
  8. Foxo1, Foxo3, and Foxo4 transcriptional network regulates autophagy and the ubiquitin-proteasome system during muscle atrophy. PMID: 25858807
  9. Liver-specific ablation of FoxO1,FoxO3 and FoxO4 prevents the induction of glucose-6-phosphatase and the repression of glucokinase during fasting, thus increasing lipogenesis. PMID: 25307742
  10. FOXO1, FOXO3a and FOXO4, are indispensable for SIRT1-dependent cell survival against oxidative stress. PMID: 24040102
  11. FoxO1/3/4 transcription factors play important roles in hepatic glucose homeostasis. PMID: 24015318
  12. Mice lacking Foxo1, -3, and -4 in bipotential progenitors of osteoblast and adipocytes exhibit increased osteoblast number and high bone mass. PMID: 23867625
  13. Foxk1 promotes muscle progenitor cell proliferation by repressing Foxo4 transcriptional activity and inhibits myogenic differentiation by repressing Mef2 activity. PMID: 22956541
  14. Genetic ablation of the 3 genes encoding FoxO isoforms 1, 3a, and 4, in myeloid cells resulted in an expansion of the granulocyte/monocyte progenitor compartment and was associated with increased atherosclerotic lesion formation. PMID: 23420833
  15. FoxO4 inhibits atherosclerosis through bone marrow derived cells, possibly by inhibition of reactive oxygen species and inflammatory cytokines that promote monocyte recruitment and/or retention. PMID: 22005198
  16. In NIH3T3 cells, tetrahydrocurcumin induced nuclear accumulation of FOXO4 PMID: 22156377
  17. Data show that glomeruli isolated from diabetic db/db mice have increased acetylation of Foxo4, suppressed expression of Sirt1, and increased expression of Bcl2l11 compared to non-diabetic littermates. PMID: 21858169
  18. Foxo4 may be a useful target for suppression in the treatment of HBV-associated hepatocellular carcinoma cells. PMID: 21567078
  19. Data indicate that FoxOs work in concert to regulate multiple aspects of hepatic glucose metabolism. PMID: 20880840
  20. a conserved critical Ku70 role for FOXO function toward coordination of a survival program PMID: 20570964
  21. Using transcriptional assays, we observed that Fhl2, in a dose-dependent fashion, promotes Foxk1 transcriptional repression of Foxo4 activity. PMID: 20013826
  22. The forkhead transcription factor AFX activates apoptosis by induction of the BCL-6 transcriptional repressor. PMID: 11777915
  23. AFX (a Forkhead transcription factor = Foxo4) is expressed in the rodent ovary PMID: 11875118
  24. plays an important role in the regulation of GADD45 in response to oxidative stress and thereby contributes to G(2)-M checkpoint PMID: 12048180
  25. the MLL-AFX fusion protein requires the transcriptional effector domains of AFX to transform myeloid progenitors and interferes with forkhead protein function PMID: 12192052
  26. mechanism for oncogenic activation of MLL by forkhead family proteins designated FKHRL1 and AFX PMID: 12393557
  27. AGE-RAGE interaction contributes to podocyte apoptosis by activation of the FOXO4 transcription factor. PMID: 17667983
  28. FoxO factors mediate Myc-induced Arf expression and provide direct genetic evidence for their tumor-suppressive capacity. PMID: 17974917
  29. Mdm2 induces mono-ubiquitination of FOXO4 PMID: 18665269
  30. The effect of tumor necrosis factor on atrogin-1 expression via Foxo protein metabolism is reported. PMID: 18701653
  31. A link between AMPK, glucose clearance, foxo4 and the leptin axis, is suggested. PMID: 19410631
  32. FoxO4 inhibits NF-kappaB and protects mice against colonic injury and inflammation. PMID: 19560465
  33. FOXO proteins enforce DNA damage-induced cell cycle arrest in hematopoietic cells. PMID: 19671690

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Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Strongly expressed in brown adipose tissue and weakly in white adipose tissue (at protein level). Expressed in skeletal muscle.
Database Links
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