Recombinant Mouse Isthmin-1 (Ism1) gets expressed in a yeast system and covers the full length of the mature protein, spanning amino acids 30 to 454. The protein comes with an N-terminal 6xHis tag, which makes purification and detection much easier. Purity levels reach above 90%, confirmed through SDS-PAGE analysis. This appears to ensure reliable performance for research work. The product is prepared under strict quality standards, which likely makes it appropriate for different experimental conditions.
Isthmin-1 (Ism1) has drawn attention in biological research because it seems to be involved in cellular processes like cell adhesion and angiogenesis. The protein may play an important role in regulating these pathways, helping scientists understand tissue development and repair mechanisms better. Researchers often work with Ism1 to explore how it functions and what it interacts with. This provides valuable insights into its significance in both normal physiology and disease states.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies
This recombinant mouse Ism1 protein works well in pull-down assays for identifying potential binding partners or interacting proteins. The N-terminal 6xHis tag allows for immobilization on nickel-affinity resins, making it possible to capture associated proteins from cell lysates or tissue extracts. These studies could help clarify the molecular mechanisms behind Ism1 function in various biological processes. The high purity (>90%) suggests reliable results with minimal background interference from contaminating proteins.
2. Antibody Development and Validation
The recombinant Ism1 protein appears to be an ideal antigen for generating specific antibodies against mouse Ism1. Scientists can use this protein to immunize animals for polyclonal antibody production or as a screening antigen for monoclonal antibody development. The protein also works for validating antibody specificity through techniques like ELISA, Western blotting, or surface plasmon resonance. The 6xHis tag offers an additional epitope for detection and purification monitoring during antibody characterization.
3. Structural and Biochemical Characterization
This full-length mature Ism1 protein (amino acids 30-454) can be used for biophysical studies to understand its structural properties and stability. Scientists can perform techniques such as circular dichroism spectroscopy, dynamic light scattering, or analytical ultracentrifugation to characterize protein folding, oligomerization state, and thermal stability. The yeast expression system may provide proper protein folding with potential post-translational modifications that are relevant to the native protein structure.
4. Cell-Based Functional Assays
The recombinant Ism1 protein can be added to cell culture systems to study its effects on cellular processes such as proliferation, differentiation, or migration. Scientists can treat various mouse cell lines with different concentrations of the protein to observe dose-dependent responses. The protein's stability and purity make it suitable for time-course experiments and co-culture studies. Such assays might provide insights into Ism1's role in cellular signaling pathways and tissue development.
