Recombinant Mouse LIM and senescent cell antigen-like-containing domain protein 1(Lims1)

Code CSB-EP859129MO
Size US$2632
Image
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP859129MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Lims1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP859129MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Lims1.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Lims1
Uniprot No. Q99JW4
Research Area Cancer
Alternative Names Lims1; Pinch1LIM and senescent cell antigen-like-containing domain protein 1; Particularly interesting new Cys-His protein 1; PINCH-1
Species Mus musculus (Mouse)
Source E.coli
Expression Region 2-325aa
Target Protein Sequence ANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGDVVSALNKAWCVSCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLSETLGRK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 37.1 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.
Gene References into Functions
  1. findings suggest that PINCH-1 regulates integrin-mediated adhesion of keratinocytes through the interactions with ILK as well as EPLIN. PMID: 25609703
  2. Rsu-1 expression is dramatically decreased in PINCH double knockout mouse livers. PMID: 24058607
  3. PINCH is increased and binds to hp-Tau. These studies address a new mechanism by which AD and HIV may intersect and identify PINCH as a contributing factor to the accumulation of hyperphosphorylated Tau. PMID: 23554879
  4. PINCH-1 inhibits JNK-mediated apoptosis by stabilising the PINCH-1 binding protein RSU-1 and promotes Bcl-2-dependent pro-survival signalling downstream of integrins. PMID: 22946061
  5. Data suggest that the adapter protein PINCH1 critically participates in the regulation of the cellular radiosensitivity of normal and malignant cells similarly under adhesion and suspension conditions. PMID: 20927395
  6. PINCH1 plays an essential role in early murine embryonic development but is dispensable in ventricular cardiomyocytes. PMID: 15798193
  7. These results provide important evidence for a crucial role of the PINCH-1-ILK-alpha-parvin complex in the control of podocyte adhesion, morphology, and survival. PMID: 15872073
  8. LIM 5 domain of PINCH1 interacts with Rsu-1 in mammalian cells and functions, in part, by altering cell adhesion. PMID: 15878342
  9. The PINCH1 is composed of 5 LIM domains, binds ILK and locates to integrin-mediated adhesion sites, and PINCH1 is for integrin function, actin organization, cell-cell adhesion and endodermal cell survival during the implanting of mouse embryos. PMID: 15976450
  10. PICH1, Ilk and alpha-parvin form a complex to costameres in neonatal mouse ventricular myocytes. This complex is stimulated by fibronectins and phenylephrine, and by both by drug synergism, to regulate cardiac myocyte hypertrophy. PMID: 16170337
  11. LIM1 domain only of either PINCH1 or PINCH2 can prevent ILK degradation despite their failure to localize to focal adhesions. PMID: 16317048
  12. Transactivation studies and chromatin immunoprecipitation implicate Lims1 as a direct target of NF-E2 regulation PMID: 17047147
  13. Pinch1 plays an essential role in neural crest development, in part through transforming growth factor-beta signaling. PMID: 17272814
  14. mechanical stretch prevented vascular smooth muscle from apoptosis via a mechanism that involves alphaVbeta3 integrin expression, stabilization of PINCH-1, and remodeling of the cytoskeleton. PMID: 17878168
  15. Results describe the roles of the ILK-PINCH-parvin triad proteins in the maturation of focal adhesions. PMID: 19435803
  16. Absence of either PINCH1 or PINCH2 in myocardium leads to exacerbated cardiac injury and deterioration in cardiac function after myocardial infarction. PMID: 19652092

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Subcellular Location Cell junction, focal adhesion, Cell membrane, Peripheral membrane protein, Cytoplasmic side
Database Links

KEGG: mmu:110829

STRING: 10090.ENSMUSP00000020077

UniGene: Mm.402697

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