Recombinant Mouse Neuron navigator 3 (Nav3) gets produced in an E. coli expression system. The protein includes a partial sequence spanning amino acids 77 to 184. An N-terminal 6xHis-tag is attached to help with purification and detection. SDS-PAGE analysis confirms the protein reaches over 85% purity, which appears to ensure reliable performance in research applications.
Neuron navigator 3 (Nav3) is involved in neuronal development and guidance. The protein seems to play a crucial role in signaling pathways that regulate axon guidance and neural circuit formation. This makes it an important focus in neurobiological research. Understanding how Nav3 functions and gets regulated may provide insights into neural network establishment and its implications in developmental neuroscience.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Antibody Development and Validation
This recombinant Nav3 fragment (77-184aa) can work as an immunogen for generating specific antibodies against mouse Nav3 protein. The N-terminal His-tag helps with purification and immobilization during antibody screening assays. Researchers might use this defined protein fragment to develop monoclonal or polyclonal antibodies that specifically recognize this Nav3 region. The purified protein also works in competitive ELISA assays to validate antibody specificity and determine binding affinity.
2. Protein-Protein Interaction Studies
Pull-down assays can make use of the His-tagged Nav3 fragment to identify potential binding partners that interact with this specific protein region. The His-tag allows easy immobilization on nickel-affinity resins, which then capture interacting proteins from cell lysates or purified protein libraries. This approach may help clarify the molecular mechanisms underlying Nav3 function by mapping protein interactions within the 77-184 amino acid region.
3. Structural and Biochemical Characterization
This recombinant protein fragment appears suitable for biophysical studies aimed at understanding the structural properties of the Nav3 protein region spanning amino acids 77-184. Circular dichroism spectroscopy, dynamic light scattering, or NMR studies can characterize the secondary structure and folding properties of this domain. The high purity (>85%) and defined boundaries likely make it appropriate for detailed biochemical analysis and structure-function relationship studies.
4. ELISA-Based Binding Assays
The His-tagged Nav3 fragment works as either a capture or detection reagent in enzyme-linked immunosorbent assays. These assays can study binding interactions with other proteins, small molecules, or nucleic acids. The His-tag allows oriented immobilization on nickel-coated plates, which ensures consistent protein presentation. This application is particularly useful for screening potential binding partners or investigating the binding kinetics of known interactors with this specific Nav3 region.
