Recombinant Mouse Nuclear respiRatory factor 1(Nrf1)

Code CSB-YP016076MOa2
Size US$1916
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Nrf1
Uniprot No. Q9WU00
Research Area Epigenetics and Nuclear Signaling
Alternative Names Nrf1; Nuclear respiratory factor 1; NRF-1; Alpha palindromic-binding protein; Alpha-pal
Species Mus musculus (Mouse)
Source Yeast
Expression Region 1-503aa
Target Protein Sequence MEEHGVTQTEHMATIEAHAVAQQVQQVHVATYTEHSMLSADEDSPSSPEDTSYDDSDILNSTAADEVTAHLAAAGPVGMAAAAAVATGKKRKRPHVFESNPSIRKRQQTRLLRKLRATLDEYTTRVGQQAIVLCISPSKPNPVFKVFGAAPLENVVRKYKSMILEDLESALAEHAPAPQEVNSELPPLTIDGIPVSVDKMTQAQLRAFIPEMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATTTHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 69.6kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication (By similarity).
Gene References into Functions
  1. mRNA and protein levels of Nrf1D were detected to varying extents in hemopoietic and somatic tissues. Nrf1D-derived isoforms were present in blood plasma. PMID: 30261697
  2. Study identifies the endoplasmic reticulum (ER)-bound transcription factor nuclear factor erythroid 2 related factor-1, Nrf1/Nfe2L1, as a critical mediator of this process and shows that Nrf1 directly binds to and specifically senses cholesterol in the ER through a defined domain and that cholesterol regulates Nrf1 turnover, processing, localization, and activity. PMID: 29149604
  3. data highlight a precise crosstalk between transcriptional regulation by NRF1 and epigenetic modulation during germ cell development and unequivocally demonstrate a novel role of NRF1 in spermatogenesis PMID: 28754714
  4. Nrf1 knockdown suppressed the death of ubiquitin-deficient N2a cells upon exposure to As(III). Therefore, the levels of p65-Nrf1 may play an important role in the maintenance of cell viability under oxidative stress induced by As(III). PMID: 28237703
  5. findings suggest that neurodegeneration in Nrf1 NKO mice may stem from the dysfunction of the ubiquitin-mediated regulation of neuronal proteins PMID: 28088524
  6. Our findings demonstrate that DEE and a fraction derived from this extract exerts anti-inflammatory effects through Nrf2dependent HO-1 expression PMID: 26647788
  7. NRF1 (nuclear respiratory factor 1) is a methylation-sensitive transcription factor; in the absence of DNA methylation, NRF1 binds to new sites and induces aberrant transcription PMID: 26675734
  8. Nrf1 plays critical roles in regulating glucose metabolism, mitochondrial function, and insulin secretion, suggesting that Nrf1 may be a novel target to improve the function of insulin-secreting beta-cells. PMID: 25556857
  9. lysine-specific demethylase 1 promotes oxidative phosphorylation in white adipose tissue, in cooperation with Nrf1. PMID: 24912735
  10. Results show that Nrf1 may not be directly responsible for the loss of Nrf2-dependent inducibility of antioxidant and cytoprotective genes observed in aged animals. PMID: 24024152
  11. Nrf1 controls both the fatty acid and the cystine/cysteine content of hepatocytes by participating in an elaborate regulatory network. PMID: 25092871
  12. Data indicate both Nrf1a and Nrf1b isoforms transcripts were detected in different mouse and human cell lines, and in various mouse tissues. PMID: 23144760
  13. During fetal myogenesis, Pitx2/3 control this redox state through the regulation of Nrf1 and of antioxidant pathways. PMID: 24871946
  14. NST-adjoining TADs are partially repartitioned out of membranes into the cyto/nucleoplasmic side, where Nrf1 is subject to deglycosylation and/or proteolysis to generate 95-kDa and 85-kDa isoforms PMID: 23774320
  15. NRF-2 and NRF-1 operate in a concurrent and parallel manner in mediating the tight coupling between energy metabolism and neuronal activity at the molecular level. PMID: 23085505
  16. Nrf1 binds to the antioxidant response elements (AREs) in regulatory regions of the Lipin1 and PGC-1beta genes and the binding of Nrf1 to the AREs activates reporter gene transcription. PMID: 22586274
  17. these results clearly suggest that both beta-TrCP- and Hrd1-dependent degradation mechanisms regulate the transcriptional activity of Nrf1 to maintain cellular homeostasis. PMID: 21911472
  18. These results show that Nrf1 sustains the CNS homeostasis through regulating target genes distinct from those regulated by Nrf2. PMID: 21554501
  19. Nrf1 is a key transcriptional regulator required for the expression of proteasomal genes in neurons and perturbations of Nrf1 function may contribute to the pathogenesis of neurodegenerative diseases PMID: 21536885
  20. Study observed that Nrf1 overexpression stimulated cell proliferation, apoptosis, and cytokine expression. Prolonged Nrf1 induced an adipokine expression profile of insulin resistant adipocytes. PMID: 21053274
  21. Findings indicate that TLR4-dependent NFkappaB and CREB activation co-regulate the NRF1 promoter with NFkappaB intronic enhancement and redox-regulated nuclear translocation, leading to downstream target-gene expression. PMID: 20587593
  22. NRF-1 overexpression sensitizes cells to apoptosis on serum depletion PMID: 12533512
  23. The Nrf1 metabolic pathway is the major regulator of genes that function in the mitochondrion. PMID: 12849846
  24. NRF-1 and NRF-2 prove to be the two key bigenomic coordinators for transcriptional regulation of all cytochrome c oxidase subunits in neurons PMID: 18077450
  25. beneficial effects of Sirt1 are due to at least two mechanisms: induction of antioxidant proteins MnSOD and Nrf1 PMID: 18599449
  26. In this study they asked whether the interactions between USF1/USF2 and E-Box and Nrf1 and GC-Box in the brain of mouse are influenced by postnatal age and what link it has with reported age-related decrease in the expression of Fmr-1 gene. PMID: 18782566
  27. Accumulation of nuclear NRF-1 protein leads to gene activation for heart mitochondrial biogenesis. PMID: 18845810
  28. NRF-1 can also directly interact with poly(ADP-ribose) polymerase 1 (PARP-1) and co-purify the PARP-1.DNA-PK.Ku80.Ku70.topoisomerase IIbeta-containing protein complex. PMID: 19181665

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Subcellular Location Nucleus
Protein Families NRF1/Ewg family
Tissue Specificity Widely expressed in embryonic, fetal, and adult tissues.
Database Links

KEGG: mmu:18181

STRING: 10090.ENSMUSP00000065568

UniGene: Mm.259258

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