Recombinant Mouse Phenylethanolamine N-methyltransferase (Pnmt)

Code CSB-YP018274MO
MSDS
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Source Yeast
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Code CSB-EP018274MO
MSDS
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Source E.coli
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Code CSB-EP018274MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018274MO
MSDS
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Source Baculovirus
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Code CSB-MP018274MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pnmt
Uniprot No.
Alternative Names
Pnmt; Phenylethanolamine N-methyltransferase; PNMTase; EC 2.1.1.28; Noradrenaline N-methyltransferase
Species
Mus musculus (Mouse)
Expression Region
1-295
Target Protein Sequence
MNGGSDLKHA TGSGSDPKHA AEMDPDSDAG QVAVALAYQR FEPRAYLRNN YAPPRGDLSN PDGVGPWKLR CMAQVFATGE VSGRVLIDIG SGPTIYQLLS ACAHFEDITM TDFLEVNRQE LGLWLREEPG AFDWSVYSQH ACLIEDKGES WQEKERQLRA RVKRVLPIDV HKPQPLGTPS LVPLPADALV SAFCLEAVSP DLTSFQRALH HITTLLRPGG HLLLIGALEE SWYLAGEARL SVVPVSEEEV REALVLGGYE VRELRTYIMP AHLCTGVDDV KGIFFAWAQK MEVQV
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Converts noradrenaline to adrenaline.
Gene References into Functions
  1. Data suggest that Pnmt plays role in fear response and startle reactivity; compared with wild-type mice, Pnmt-knockout mice exhibit reduced contextual fear but normal cued fear. PMID: 23268986
  2. Although temperature was normal in Pnmt(-/-) mice, the brown fat response to cold was abnormal with no increase in Ucp-1 or Pgc-1alpha mRNA levels (but with an exaggerated cold-induced lipid loss from the tissue). PMID: 20619316
  3. surprisingly high levels of the epinephrine synthesizing enzyme phenylethanolamine N-methyl transferase (PNMT) were detected in the thymus of young mice. PMID: 12096893
  4. A truncated mouse phenylethanolamine N-methyltransferase splice variant has dominant-negative activity PMID: 12438096
  5. PNMT expression is regulated late in mouse gestation by AP2-alpha and glucocorticoids PMID: 12438097
  6. Glucocorticoid receptors form multimers of Pmnt independent of the DNA binding domain. PMID: 12933902
  7. clear, significant increase in aggression arising from PNMT overexpression PMID: 15824970
  8. Regulation of the PNMT gene expression in various compartments of heart includes both corticosterone-dependent and independent mechanisms. PMID: 16691441
  9. Transcriptional suppression by cAMP as a mechanism for regulating PNMT expression in both normal and neoplastic mouse chromaffin cells. PMID: 17196587
  10. Epinephrine is required for normal blood pressure and cardiac filling responses to stress but is not required for tachycardia during stress or normal cardiovascular function at rest in the knockout mouse. PMID: 17698731
  11. Neuronal nitric oxide synthase gene inactivation reduces the expression of vasopressin in the hypothalamic paraventricular nucleus and of PNMT in the adrenal gland of the mouse. PMID: 17853069

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Protein Families
Class I-like SAM-binding methyltransferase superfamily, NNMT/PNMT/TEMT family
Tissue Specificity
Brain (pons and medulla oblongata), adrenal gland and retina.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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