Recombinant Mouse Proteasome subunit beta type-10 (Psmb10)

Code CSB-YP018877MO
MSDS
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Source Yeast
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Code CSB-EP018877MO
MSDS
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Source E.coli
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Code CSB-EP018877MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018877MO
MSDS
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Source Baculovirus
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Code CSB-MP018877MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Psmb10
Uniprot No.
Alternative Names
Psmb10; Lmp10; Mecl1; Proteasome subunit beta type-10; EC 3.4.25.1; Low molecular mass protein 10; Macropain subunit MECl-1; Multicatalytic endopeptidase complex subunit MECl-1; Proteasome MECl-1; Proteasome subunit beta-2i
Species
Mus musculus (Mouse)
Expression Region
40-273
Target Protein Sequence
T TIAGLVFRDG VILGADTRAT NDSVVADKSC EKIHFIAPKI YCCGAGVAAD TEMTTRMAAS KMELHALSTG REPRVATVTR ILRQTLFRYQ GHVGASLVVG GVDLNGPQLY EVHPHGSYSR LPFTALGSGQ GAAVALLEDR FQPNMTLEAA QELLVEAITA GILSDLGSGG NVDACVITAG GAKLQRALST PTEPVQRAGR YRFAPGTTPV LTREVRPLTL ELLEETVQAM EVE
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Plays a role in determining the T-cell repertoire for an antiviral T-cell response.
Gene References into Functions
  1. Our results showed that immunoproteasome subunit LMP10 expression and its trypsin-like activity were significantly upregulated in the retinas and serum of Ang II-infused mice and in the serum from patients with hypertensive retinopathy..this study provided novel evidence demonstrating that LMP10 is a positive regulator of NF-kB signaling, which contributes to Ang II-induced retinopathy. PMID: 29499566
  2. The knockout of beta2i ameliorates DOCA/salt-induced cardiac fibrosis and inflammation, and may be a novel potential therapeutic target for hypertensive heart diseases. PMID: 28478040
  3. phenotype of hyperproliferation of T cells lacking both MECL-1 and LMP7 implicates a specific role for immunoproteasomes in T cell proliferation that is not obviously connected to MHC class I Ag processing PMID: 16547243
  4. An in vivo function of MECL-1 codetermines the T cell repertoire for an antiviral cytotoxic T lymphocyte (CTL) response specific for lymphocytic choriomeningitis virus that is altered in MECL1-deficient mice. PMID: 16709825
  5. The findings indicate that MECL-1 influences the homeostatic equilibrium between T-cell subsets, not through indirect extracellular signals, such as MHC-I expression or the cytokine milieu, but through direct effects on T-cell-intrinsic processes. PMID: 18160473

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Peptidase T1B family
Tissue Specificity
Detected in liver (at protein level).
Database Links

KEGG: mmu:19171

STRING: 10090.ENSMUSP00000034369

UniGene: Mm.787

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