Product Details

Purity

>85% (SDS-PAGE)

Target Names

Arhgef9

Uniprot No.

Q3UTH8

Alternative Names

Arhgef9; Kiaa0424Rho guanine nucleotide exchange factor 9; Collybistin; Rac/Cdc42 guanine nucleotide exchange factor 9

Species

Mus musculus (Mouse)

Expression Region

1-516

Target Protein Sequence

MYDLITGDSI VSAEAVWDHV TMANRELAFK AGDVIKVLDA SNKDWWWGQI DDEEGWFPAS FVRLWVNQED GVEEGPSDVQ NGHLDPNSDC LCLGRPLQNR DQMRANVINE IMSTERHYIK HLKDICEGYL KQCRKRRDMF SDEQLKVIFG NIEDIYRFQM GFVRDLEKQY NNDDPHLSEI GPCFLEHQDG FWIYSEYCNN HLDACMELSK LMKDSRYQHF FEACRLLQQM IDIAIDGFLL TPVQKICKYP LQLAELLKYT AQDHSDYRYV AAALAVMRNV TQQINERKRR LENIDKIAQW QASVLDWEGE DILDRSSELI YTGEMAWIYQ PYGRNQQRVF FLFDHQMVLC KKDLIRRDIL YYKGRIDMDK YEVIDIEDGR DDDFNVSMKN AFKLHNKETE EVHLFFAKKL EEKIRWLRAF REERKMVQED EKIGFEISEN QKRQAAMTVR KASKQKGVNS ARSVPPSYPP PQDPLNQGQY LVPDGIAQSQ VFEFTEPKRS QSPFWQNFSR LTPFKK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters.

Gene References into Functions

Collybistin binds the GABAAR-alpha2-subunit with high affinity PMID: 30087324
Results uncover previously unexpected role for CB isoforms downstream of alpha2-containing GABAARs during neuron maturation in a Cdc42 dependent mechanism. PMID: 29069083
Within this network, collybistin can adopt open/active and closed/inactive conformations to act as a switchable adaptor that links gephyrin to plasma membrane phosphoinositides. PMID: 25082542
data show that collybistin co-clusters with gephyrin and GABA(A) Rs in synaptic puncta and is recruited to postsynaptic specializations early during synapse development; it co-localizes with GABA(A) Rs containing the alpha1, alpha2, or alpha3 subunits PMID: 21681748
The results of this study provided evidence that the formation of gephyrin scaffolds at inhibitory synapses requires an intact Cb II PH-domain but is Cdc42-independent. PMID: 20345913
The resolution crystal structure of the Cdc42-collybistin II complex reveals a novel conformation of the switch I region of Cdc42, and biochemical data indicate that gephyrin negatively regulates collybistin activity. PMID: 16616186
Cb is essential for gephyrin-dependent clustering of a specific set of GABA(A) receptors, but not required for glycine receptor postsynaptic localization. PMID: 17690689
Data provide the first evidence that collybistin deficiency leads to significant changes of GABAergic inhibition, network excitability and synaptic plasticity in vivo. PMID: 19236916

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Subcellular Location

Cytoplasm. Cell junction, synapse, postsynaptic density.

Tissue Specificity

Detected in embryonic and adult brain.

Database Links

KEGG: mmu:236915

STRING: 10090.ENSMUSP00000109510

UniGene: Mm.44841

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Recombinant Mouse Rho guanine nucleotide exchange factor 9 (Arhgef9)

Product Details

Customer Reviews and Q&A

Target Background

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