Recombinant Mouse Survival motor neuron protein (Smn1) is produced using a mammalian cell expression system, which appears to ensure proper eukaryotic post-translational modifications and functionality. This full-length protein spans amino acids 1-288 and includes an N-terminal 10xHis tag plus a C-terminal Myc tag for purification and detection purposes. SDS-PAGE analysis indicates the protein reaches purity levels exceeding 85%, which should make it suitable for various research applications.
The survival motor neuron protein (Smn1) seems to play a critical role in assembling small nuclear ribonucleoproteins (snRNPs) - key components of the spliceosome complex. It participates in pre-mRNA splicing, a fundamental process in gene expression. Smn1's function is likely vital for cellular RNA processing and has become a focal point for understanding molecular mechanisms of RNA metabolism and related cellular pathways.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies Using Pull-Down Assays
The dual tagging system - that N-terminal 10xHis tag paired with the C-terminal Myc tag - allows for efficient purification and detection of Smn1 protein complexes. Researchers can immobilize this dual-tagged recombinant Smn1 on nickel-affinity resins to capture interacting proteins from mouse tissue lysates or cell extracts. The Myc tag then provides a way to specifically detect and confirm Smn1 presence in pulled-down complexes using anti-Myc antibodies. This approach may prove particularly valuable for identifying novel binding partners of the survival motor neuron protein in motor neuron biology research.
2. Antibody Development and Validation
This full-length recombinant mouse Smn1 protein could serve as an ideal antigen for generating specific antibodies against the survival motor neuron protein. The mammalian expression system likely ensures proper protein folding and post-translational modifications that closely resemble the native protein. Researchers might use this protein to immunize animals for polyclonal antibody production or as a screening antigen for monoclonal antibody development. Those dual tags also offer a way to validate antibody specificity through tag-based detection controls.
3. Biochemical Characterization and Stability Studies
This purified recombinant Smn1 protein enables detailed biochemical analysis - think protein stability assessments, folding property studies, and buffer optimization experiments. Thermal stability assays, pH tolerance tests, and storage condition optimization all become possible with this full-length protein. The 85% purity level appears sufficient for most biochemical characterization experiments, allowing researchers to investigate how the protein behaves under various experimental conditions relevant to motor neuron research.
4. ELISA-Based Quantitative Assays
The dual-tagged Smn1 protein may serve as a standard or control in enzyme-linked immunosorbent assays for quantifying Smn1 levels in biological samples. Both Myc and His tags provide multiple detection options, which could enable sandwich ELISA formats or direct detection methods. This application might prove particularly useful for researchers studying Smn1 expression levels across different mouse tissues, developmental stages, or disease models where quantitative measurement of the survival motor neuron protein is required.
