Recombinant Mouse Transcription factor LBX1 (Lbx1)

Code CSB-YP012777MO
MSDS
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Source Yeast
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Code CSB-EP012777MO
MSDS
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Source E.coli
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Code CSB-EP012777MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP012777MO
MSDS
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Source Baculovirus
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Code CSB-MP012777MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lbx1
Uniprot No.
Alternative Names
Lbx1; Lbx1h; Transcription factor LBX1; Ladybird homeobox protein homolog 1
Species
Mus musculus (Mouse)
Expression Region
1-282
Target Protein Sequence
MTSKEDGKAA PGEERRRSPL DHLPPPANSN KPLTPFSIED ILNKPSVRRS YSLCGAAHLL AAADKHAPGG LPLAGRALLS QTSPLCALEE LASKTFKGLE VSVLQAAEGR DGMTIFGQRQ TPKKRRKSRT AFTNHQIYEL EKRFLYQKYL SPADRDQIAQ QLGLTNAQVI TWFQNRRAKL KRDLEEMKAD VESAKKLGPS GQMDIVALAE LEQNSEASGG GGGGGCGRAK SRPGSPALPP GAPQAPGGGP LQLSPASPLT DQRASSQDCS EDEEDEEIDV DD
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor required for the development of GABAergic interneurons in the dorsal horn of the spinal cord and migration and further development of hypaxial muscle precursor cells for limb muscles, diaphragm and hypoglossal cord.
Gene References into Functions
  1. Chmp2b is one of the most highly regulated cell-autonomous targets of Lbx1 in the embryonic mouse neural tube PMID: 23284619
  2. Lbx1 is expressed in and required for the correct specification of three early dorsal interneuron populations and late-born neurons that form the substantia gelatinosa. Lbx1 plays a critical role in the development of sensory pathways in the spinal cord. PMID: 12062038
  3. Lbx1 is required for correct dorsal-ventral patterning of the neural tube. Lbx1/Pax3 double-mutant mice showed a completely open neural tube. PMID: 12358782
  4. Inactivation of the Lbx1 gene in mice resulted in defects in heart looping, changes in gene expression pattern, and increased cell division; Pax3 and Lbx1 participate in negative regulatory feedback PMID: 12522123
  5. Corl1 interacted with Lbx1 and cooperatively repressed transcription, suggesting that it acts as a transcriptional corepressor for Lbx1 in regulating cell fate determination in the dorsal spinal cord. PMID: 15528197
  6. Lbx1 and Tlx3 are opposing switches in determining GABAergic versus glutamatergic transmitter phenotypes. PMID: 16234809
  7. Homeobox gene Lbx1 is essential for imposing a somatosensory fate on relay neurons in the hindbrain. PMID: 17475798
  8. Functional analysis of homeodomain-containing transcription factor Lbx1 in satellite cells of mouse skeletal muscle is reported. PMID: 18003701
  9. The respiratory rhythm generation within the pre-Botzinger complex (preBotC) is presumably functional in Lbx1 mutant mice with additional neurochemical drive. PMID: 18945911

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the dorsal part of the spinal cord and hindbrain and in presumptive myogenic cells in lateral regions of differentiating somites.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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