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Recombinant Mouse Troponin I, slow skeletal muscle (Tnni1)

Recombinant Mouse Troponin I, slow skeletal muscle (Tnni1)

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Code	CSB-EP895293MO
Abbreviation	Recombinant Mouse Tnni1 protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Tnni1

Uniprot No.

Q9WUZ5

Research Area

Signal Transduction

Alternative Names

Tnni1; Troponin I; slow skeletal muscle; Troponin I; slow-twitch isoform

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

2-187aa

Target Protein Sequence

PEVERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

29.6 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-Flag-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Expressing the recombinant Mouse Tnni1 protein in e.coli cells involves inserting a DNA fragment encoding the Mouse Tnni1 protein (2-187aa) into a plasmid vector and transferring it to the e.coli cells. Positive cells are screened, cultured, and induced to express the Tnni1 protein. The protein carries a N-terminal 10xHis-Flag tag and C-terminal Myc tag. The cells are lysed to collect the recombinant Mouse Tnni1 protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Mouse Tnni1 protein exceeds 85%.

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Target Background

Function

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Gene References into Functions

data demonstrate that thyroid hormone can alter the time course of ssTnI gene expression in cultured cardiac myocytes and TnI gene regulation is also controlled by some unknown programmed events inside of cardiac myocytes PMID: 15695175
a localized N-terminal region of TnI comprised of amino acids 33-80, which interacts with C-terminal regions of cTnC and cTnT, is of particular significance in transducing signaling of thin filament activation by strong cross-bridges PMID: 17099250
K+-depolarization increased Tnni1 and Acta1 mRNA levels in both differentiated C2C12 and rat skeletal muscle cells in primary culture PMID: 17146758
the up-stream domain ( approximately 1,800 bp) of mouse fetal TnI gene is been cloned and characterized PMID: 18357515
Phosphorylation of troponin I was significantly reduced in Galphaq-overexpressing mice compared to wild type. PMID: 18473122

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Protein Families

Troponin I family

Database Links

KEGG: mmu:21952

STRING: 10090.ENSMUSP00000121343

UniGene: Mm.44379

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