Recombinant Plasmodium falciparum Multidrug resistance protein (MDR1), partial

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Code CSB-EP318386PLT
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
MDR1
Uniprot No.
Research Area
Others
Alternative Names
MDR1; Multidrug resistance protein; EC 7.6.2.2; Chloroquine resistance protein
Species
Plasmodium falciparum (isolate FC27 / Papua New Guinea)
Source
E.coli
Expression Region
1084-1419aa
Target Protein Sequence
KGDSENAKLSFEKYYPLMIRKSNIDVRDDGGIRINKNLIKGKVDIKDVNFRYISRPNVPIYKNLSFTCDSKKTTAIVGETGSGKSTFMNLLLRFYDLKNDHIILKNDMTNFQDYQNNNNNSLVLKNVNEFSNQSGSAEDYTVFNNNGEILLDDINICDYNLRDLRNLFSIVSQEPMLFNMSIYENIKFGREDATLEDVKRVSKFAAIDEFIESLPNKYDTNVGPYGKSLSGGQKQRIAIARALLREPKILLLDEATSSLDSNSEKLIEKTIVDIKDKADKTIITIAHRIASIKRSDKIVVFNNPDRNGTFVQSHGTHDELLSAQDGIYKKYVKLAK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
54.2kDa
Protein Length
Cytoplasmic Domain
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Amino acids 1084-1419 constitute the expression domain of recombinant Plasmodium falciparum MDR1. The theoretical molecular weight of the MDR1 protein is 54.2 kDa. This MDR1 recombinant protein is manufactured in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of MDR1, which enables a simple process of detecting and purifying the MDR1 recombinant protein in the following steps.

Plasmodium falciparum multidrug resistance protein 1 (MDR1) is an ATP-binding cassette (ABC) transporter encoded by the pfmdr1 gene in the genome of the malaria-causing parasite, Plasmodium falciparum. MDR1 is primarily associated with resistance to multiple antimalarial drugs. MDR1 plays a critical role in mediating the efflux of various drugs from the parasite's digestive vacuole, where hemoglobin degradation occurs during the blood stage of the Plasmodium life cycle. The overexpression or mutations in the pfmdr1 gene can lead to reduced drug accumulation within the parasite, contributing to resistance against commonly used antimalarial drugs, including chloroquine and mefloquine. Understanding the function and regulation of MDR1 is crucial for developing strategies to overcome drug resistance and enhance the efficacy of antimalarial treatments. Researchers aim to explore new drug targets and design alternative therapeutic approaches to combat drug-resistant strains of Plasmodium falciparum.

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Target Background

Function
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily
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