Recombinant Plasmodium falciparum Multidrug resistance protein (MDR1), partial

Amino acids 1084-1419 constitute the expression domain of recombinant Plasmodium falciparum MDR1. The theoretical molecular weight of the MDR1 protein is 54.2 kDa. This MDR1 recombinant protein is manufactured in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of MDR1, which enables a simple process of detecting and purifying the MDR1 recombinant protein in the following steps.

Plasmodium falciparum multidrug resistance protein 1 (MDR1) is an ATP-binding cassette (ABC) transporter encoded by the pfmdr1 gene in the genome of the malaria-causing parasite, Plasmodium falciparum. MDR1 is primarily associated with resistance to multiple antimalarial drugs. MDR1 plays a critical role in mediating the efflux of various drugs from the parasite's digestive vacuole, where hemoglobin degradation occurs during the blood stage of the Plasmodium life cycle. The overexpression or mutations in the pfmdr1 gene can lead to reduced drug accumulation within the parasite, contributing to resistance against commonly used antimalarial drugs, including chloroquine and mefloquine. Understanding the function and regulation of MDR1 is crucial for developing strategies to overcome drug resistance and enhance the efficacy of antimalarial treatments. Researchers aim to explore new drug targets and design alternative therapeutic approaches to combat drug-resistant strains of Plasmodium falciparum.