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Recombinant Pseudomonas aeruginosa UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)

Recombinant Pseudomonas aeruginosa UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)

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Code	CSB-EP342468EZX
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP342468EZX could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) lpxC. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP342468EZX could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) lpxC.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

lpxC

Uniprot No.

P47205

Research Area

Signal Transduction

Alternative Names

lpxC; envA; PA4406; UDP-3-O-acyl-N-acetylglucosamine deacetylase; UDP-3-O-acyl-GlcNAc deacetylase; EC 3.5.1.108; UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase

Species

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Source

E.coli

Expression Region

1-303aa

Target Protein Sequence

MIKQRTLKNIIRATGVGLHSGEKVYLTLKPAPVDTGIVFCRTDLDPVVEIPARAENVGETTMSTTLVKGDVKVDTVEHLLSAMAGLGIDNAYVELSASEVPIMDGSAGPFVFLIQSAGLQEQEAAKKFIRIKREVSVEEGDKRAVFVPFDGFKVSFEIDFDHPVFRGRTQQASVDFSSTSFVKEVSRARTFGFMRDIEYLRSQNLALGGSVENAIVVDENRVLNEDGLRYEDEFVKHKILDAIGDLYLLGNSLIGEFRGFKSGHALNNQLLRTLIADKDAWEVVTFEDARTAPISYMRPAAAV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

49.4kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

Can you quote for the following?
Catalog number:EP342468EZX (N_(6xHis-SUMO)-(Full Length lpxC_P47205_1-303aa)_C)
Qty: 5 mg each
Purity: >=95%

Thanks for your inquiry. Recombinant Pseudomonas aeruginosa UDP-3-O-acyl-N-acetylglucosamine deacetylase(lpxC)
CSB-YP342468EZX >> Yeast
CSB-EP342468EZX >> E.coli
CSB-BP342468EZX >> Baculovirus
CSB-MP342468EZX >> Mammalian cell
Expression Region: 1-303aa, full length
Tag Info: EP: N-terminal 6xHis-SUMO-tag; YP, BP, MP: N-terminal 6xHis-tag
Expression Region:

Our quality control standard for semi-customized protein purity is >85% by SDS-PAGE. We could only try to improve the purity to meet your requirement, but can't guarantee that the purity can reach 95%.

Target Background

Function

Catalyzes the hydrolysis of UDP-3-O-myristoyl-N-acetylglucosamine to form UDP-3-O-myristoylglucosamine and acetate, the committed step in lipid A biosynthesis.

Protein Families

LpxC family

Database Links

KEGG: pae:PA4406

STRING: 208964.PA4406

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