Recombinant Rat Aminopeptidase B (Rnpep)

Code CSB-YP019905RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP019905RA
MSDS
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Source E.coli
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Code CSB-EP019905RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019905RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP019905RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rnpep
Uniprot No.
Alternative Names
RnpepAminopeptidase B; AP-B; EC 3.4.11.6; Arginine aminopeptidase; Arginyl aminopeptidase; Cytosol aminopeptidase IV
Species
Rattus norvegicus (Rat)
Expression Region
2-650
Target Protein Sequence
ESSGPSSCH SAARRPLHSA QAVDVASASS FRAFEILHLH LDLRAEFGPP GPGPGSRGLN GKATLELRCL LPEGASELRL DSHSCLEVMA ATLLRGQPGD QQQLTEPVPF HTQPFSHYGQ ALCVVFPKPC CAAERFRLEL TYRVGEGPGV CWLAPEQTAG KKKPFVYTQG QAVLNRAFFP CFDTPAVKCT YSALVEVPDG FTAVMSASTW ERRGPNKFFF QMSQPIPSYL IALAIGDLAS AEVGPRSRVW AEPCLIEAAK EEYNGVIEEF LATGEKLFGP YVWGRYDLLF MPPSFPFGGM ENPCLTFVTP CLLAGDRSLA DVIIHEISHS WFGNLVTNAN WGEFWLNEGF TMYAQRRIST ILFGAAYTCL EAATGRALLR QHMDVSGEEN PLNKLRVKIE PGVDPDDTYN ETPYEKGYCF VSYLAHLVGD QEQFDKFLKA YVDEFKFQSI LAEDFLEFYL EYFPELKKKG VDSIPGFEFN RWLNTPGWPP YLPDLSPGDS LMKPAEELAE LWAASEPDMQ AIEAVAISTW KTYQLVYFLD KILQKSPLPP GNVKKLGETY PKISNAQNAE LRLRWGQIIL KNDHQEEFWK VKDFLQSQGK QKYTLPLYHA MMGGSEMART LAKETFSATA SQLHSNVVNY VQQILAPKGS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4).
Gene References into Functions
  1. A structural analysis using circular dichroism, fluorescence spectroscopy, molecular modelling and dynamics was performed to investigate the role that residue G(298) plays in the catalytic mechanism of Ap-B PMID: 21237246
  2. Results suggest that the upregulation of serotonergic activity may be related to a lowered brain dipeptidyl peptidase III, alanyl- and arginyl aminopeptidase activity which may have an influence on the cleavage of their physiological substrates. PMID: 18547641
  3. Zinc regulation demonstrates the metallopeptidase property of AP-B. PMID: 18571504
Subcellular Location
Secreted.
Protein Families
Peptidase M1 family
Tissue Specificity
Widely expressed.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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