Recombinant Rat Plasminogen activator inhibitor 1(Serpine1)

Code CSB-EP021081RA
Size US$2466
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Serpine1
Uniprot No. P20961
Research Area Others
Alternative Names Serpine1; Pai1; Planh1; Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 24-402aa
Target Protein Sequence SPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTTAGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQRDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISDLLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVPMMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAITNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFSSTQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPTEMVLDRSFLFVVRHNPTETILFMGQLMEP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 58.6kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence (By similarity). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).
Gene References into Functions
  1. PAI-1 induces alveolar type II cell senescence through activating p53-p21-Rb pathway in fibrotic lung disease. PMID: 28722352
  2. PAI-1 is implicated in the pathophysiology of depression. PMID: 27456456
  3. Our results demonstrate the antifibrotic effect of PTX on radiation-induced lung fibrosis and its effect on modulation of PKA and PAI-1 expression as possible antifibrotic mechanisms. PMID: 28337441
  4. Paclitaxel at low non-cytotoxic doses ameliorates renal fibrosis by inhibiting multiple steps in the TGF-beta1-induced PAI-1 signaling including Smads and mitogen-activated protein kinases. PMID: 26869361
  5. Aldosterone at different concentrations exerts synergistic effect with TGF-beta1 to increase PAI-1 expression in hepatic stellate cells PMID: 26617694
  6. inhibition of ERK1/2 activation has no therapeutic effect on kidney fibrosis in SNx possibly due to increased compensatory activation of the p38 and JNK signalling pathways with subsequent upregulation of PAI-1. PMID: 26415098
  7. These results suggest the critical physiological role of TRL4-Fyn interaction in the modulation of PAI-1-tPA axis in astrocytes during neuroinflammatory responses such as ischemia/reperfusion injuries. PMID: 25106729
  8. these results suggested that TGF-beta1 and Serpine 1 overexpression might play an important role in DVT formation and have predictive values. PMID: 26535698
  9. PAI-1 contributes to the regulation of the inflammatory response in alveolar macrophages. PMID: 25342286
  10. Neurite extension effects of valproic acid are mediated by modulating the tPA/plasminogen activator inhibitor (PAI)-1 system. PMID: 23378038
  11. Together, because CPAI blocks both proteolytic and nonproteolytic tPA neurotoxicity, it is a promising therapeutics of neonatal HI injury either with or without infection. PMID: 22556277
  12. PAI-1 promotes the proliferation, transforming into myofibroblasts, collagen synthesis, and inhibits apoptosis of pulmonary fibroblasts by activating Ca(2+), ERK and AKT signaling pathway. PMID: 23021499
  13. adhesion scores, PAI-1 activity in peritoneal lavage fluid, and PAI-1 mRNA levels in peritoneal mesothelium were significantly greater in the open surgery group than the control and laparoscopic surgery groups PMID: 23218127
  14. This study supports that a preceding progesterone-enriched milieu prevented the enhanced prothrombotic risk induced by a fructose-rich diet-elicited high PAI-1 production. PMID: 23016136
  15. down-regulation of PAI-1 and MMP-9 might contribute to abnormal programming of renal growth in rats exposed to postnatal early overnutrition PMID: 21752621
  16. The data demonstrate that PAI-1 siRNA inhibits alveolitis and pulmonary fibrosis in bleomycin-treated rats via inhibiting the proliferation and promoting the apoptosis of fibroblasts. PMID: 22659625
  17. mRNA, protein and activity of matrix metalloproteinase (MMP)-2 and MMP-9 and mRNA of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1) were quantified in abdominal aorta (AA) and femoral artery (FA). PMID: 22071631
  18. Hydrocortisone up-regulates PAI-1 expression in a tissue-specific manner along with down-regulation of tPA activity in both normal and inflammatory conditions. PMID: 21412817
  19. The activity of tissue plasminogen activator peaks transiently and then decreases in cortex and striatum along with delayed induction of PAI-1 in the inflammatory stage after reperfusion injury. PMID: 21193004
  20. Data suggest PAI1 plays key role in modulating chromaffin cell neurosecretory function. Evidence is presented that in chromaffin cells, PAI1 is targeted to storage vesicles and co-released with catecholamine upon secretagogue stimulation. PMID: 21596853
  21. PAI-1 derived from the neurovascular unit and peripheral vascular system participates as a positive regulator of the blood-brain barrier in facilitating the barrier function of the endothelial tight junctions. PMID: 21036181
  22. Erigeron breviscapus injection can inhibit the expression of TNF-alpha and PAI-1, and increase the expression of tPA in rats with acute myocardial infarction. PMID: 21355198
  23. PAI-1 may play a protective role against cardiovascular disease in diabetes PMID: 20538356
  24. Expression of u-PA/PAI-1 mRNA and protein increased during the early stage of hyperoxic exposure and decreased during the middle and late stage. PMID: 19099788
  25. The results suggest that SHP inhibits PAI-1 expression in VSMCs through the suppression of TGF-beta/Smad3 and AP-1 activity. PMID: 19887897
  26. High glucose decreased the expressions of tPA and uPA, and increased PAI1. Losartan could partly reverse partly this expression. PMID: 17953365
  27. Dahuang Zhechong Pill can down-regulate the expressions of TIMP-1 and PAI-1 mRNAs in renal tissues of rats with focal segmental glomerulosclerosis. PMID: 18471418
  28. Inactivation of PAI-1 speeds lysis of a platelet-rich clot in rat mesenteric arterioles. Active PAI-1 participates in arteriolar microcirculation resistance to thrombolysis and its inactivation may shorten ischemic periods after microvascular obstruction. PMID: 11776323
  29. The hexosamine pathway regulates the plasminogen activator inhibitor-1 gene promoter and Sp1 transcriptional activation through protein kinase C-betaI and -delta. PMID: 12105191
  30. pathways of regulation of PAI1 induction and secretion by growth factors bFGF and PDGF in smooth muscle cells PMID: 12192306
  31. Data show that recovery from obstruction-induced renal fibrosis was correlated with diminished expression of TGF-beta, collagen IV, and plasminogen activator inhibitor-1. PMID: 12365557
  32. HIF1a and hypoxia response elements mediate the induction of PAI-1 gene expression by insulin via the PI3K/PKB pathway in primary rat hepatocytes. PMID: 12393531
  33. possible role as the neurotrophic factor PMID: 12491782
  34. Expression of functionally active PN-1 has been examined in vitro in rat aortic smooth muscle cells and in vivo in rat arterial media and its regulation in hypertensive rats. PMID: 12524238
  35. Northern analysis confirmed upregulation of PAI-1, P2X4, and P15INK4B in cultured alveolar epithelial type I cells (AT1); in whole lung, all three proteins were detected in alveolar epithelium in a location consistent with expression in AT1 cells PMID: 12600825
  36. hypoxia-dependent plasminogen activator inhibitor 1 expression is regulated by MAP kinases PMID: 12669121
  37. In cells expressing RacG12V, ROS production was increased and PAI-1 mRNA levels as well as HIF-alpha nuclear presence were reduced under normoxia and hypoxia; whereas expression of RacT17N resulted in decreased ROS production and opposite effects. PMID: 12719791
  38. Enhanced expression of clock genes may increase PAI-1 expression in concert with activated renin-angiotensin system in SHR heart PMID: 12777947
  39. after transient focal cerebral ischemia, there are time dependent changes in PAI-1 expression PMID: 14512838
  40. cellular carbonyl stress may stimulate PAI-1 synthesis in and release from adipose tissues through ROS formation PMID: 14610081
  41. Pai1 expression is required for epithelial cell migration in in vitro wound healing. PMID: 15174100
  42. PAI-1 and TGF-beta 1 in carotid glomus and autonomic ganglia were significantly increased (P < 0.01) in hypertensive rats PMID: 15201552
  43. Altered expression of Pai-1 in diabetic nephropathy was associated with mesangial expansion. PMID: 15322501
  44. Regression of glomerulosclerosis was linked to decreased tissue inhibitor of metalloproteinase-1 and plasminogen activator inhibitor-1. PMID: 15728787
  45. Cardiac PAI-1 gene expression regulation is mediated through c-Jun NH2-terminal kinase activation. PMID: 15808835
  46. the essential requirement of MAPK/AP-1 activation for TGF-beta1-induced PAI-1 expression is unique to rat mesangial cells PMID: 16105028
  47. A dual role for PAI-1 in epithelial cell wound healing, both as a soluble inhibitor of proteolysis and also as a matrix-bound regulator of cell migration, is proposed. PMID: 17071586
  48. This study indicate that aldosterone can increase PAI-1 mRNA and protein expression by cultured mesangial cells alone, which is independent of aldosterone-induced increases in TGF-beta(1) expression and cellular reactive oxygen species. PMID: 17394767
  49. Renin induces ERK1/2 activation. Renin-activated pathway triggers cell proliferation along with TGF-beta1 and plasminogen activator inhibitor-1 gene expression. PMID: 17396111
  50. PAI-1 plays a role in the occurrence of liver failure after excessive hepatectomy via accelerated maturation of pro-uPA and fibrinolytic factors. PMID: 17655862

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Subcellular Location Secreted
Protein Families Serpin family
Database Links

KEGG: rno:24617

STRING: 10116.ENSRNOP00000001916

UniGene: Rn.29367

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