Recombinant Rat Semaphorin-3A(Sema3a)

Code CSB-EP717391RA
Size US$2466Purchase it in Cusabio online store
(only available for customers from the US)
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Sema3a
Uniprot No. Q63548
Research Area Cardiovascular
Alternative Names Sema3a; Semaphorin-3A; Semaphorin III; Sema III
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 21-772aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 90.6kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function May be involved in guiding growing axons towards their targets by forming a molecular boundary that instructs axons to engage in the formation of specific nerve tracts. Binds to neuropilin. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).
Gene References into Functions
  1. decreased expression of Sema3A in bone mesenchymal stem cells from type 2 diabetes mellitus rats compared with bone mesenchymal stem cells from normal rats. PMID: 29774455
  2. decreased expression of Sema3A/Nrp1 was observed in periapical lesions and is potentially involved in the bone resorption of the periapical area, suggesting that Sema3A/Nrp1 may contribute to the pathological development of apical periodontitis PMID: 29457037
  3. describe methods to visualize endocytosed Semaphorin3A (Sema3A) molecules and to characterize the rate and pathway of internalization in primary rat neuronal cultures using semiconductor quantum dot nanoparticles PMID: 27787858
  4. describe microfabrication and substrate stripe micropatterning of Sema3A PMID: 27787862
  5. a Sema3A-initiated apoptotic signaling complex regulates the apoptosis of sympathetic neurons during the period of naturally occurring cell death during development of the peripheral nervous system. PMID: 27143756
  6. Data suggest that voltage-gated calcium and sodium channels mediate Sema3A retrograde signaling that regulates dendritic GluA2 localization and branch formation. PMID: 26638837
  7. Semaphorin 3A deficiency could improve hypoxia-induced myocardial injury via resisting inflammatory factors secretion, viability decline, cardiomyocyte apoptosis, reactive oxygen species release, ATP generation decline. PMID: 26950444
  8. Upregulation of Sema3A increased cardiac electrical stability and reduced ventricular arrhythmias (VA). Sema3A silencing lead to sympathetic hyperinnervation, increased myocardial norepinephrine content and inducible VAs. PMID: 26787044
  9. Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections. PMID: 25161316
  10. This study delineate a Semaphorin-dependent signaling pathway that promotes dendritic complexity via the actin regulatory protein Farp1. PMID: 24899721
  11. Overexpression of Sema3a in myocardial infarction border zone could reduce the inducibility of ventricular arrhythmias by reducing sympathetic hyper-reinnervation after infarction. PMID: 23711091
  12. Sema3A binding to CS-E in the PNNs may be a mechanism whereby PNNs restrict growth and plasticity and may represent a possible point of intervention to facilitate neuronal plasticity. PMID: 23940048
  13. In the early stages of O2-induced retinopathy, retinal microglia are activated to produce IL-1beta, which sustains the activation of microglia and induces microvascular injury through the release of Sema3A from adjacent neurons. PMID: 23766263
  14. Interplay of neuropilin-1 and semaphorin 3A after partial hepatectomy in rats. PMID: 23049211
  15. These new data on Class 3 Semaphorins and their receptors provide more information about the complex reactive events that occur bilaterally in the retino-collicular system following unilateral adult optic nerve injury. PMID: 23139269
  16. Cytoskeleton reorganization mediated by Sema3A is relatively slow to induce filopodia repulsion at the growth cone. PMID: 22997549
  17. Sema3a was expressed in the heart by cardiocytes. Increased expression of sema3a may partly account for sympathetic denervation in congestive heart failure. PMID: 21933622
  18. Sema3A regulates the earliest step of neuronal morphogenesis by polarizing axon/dendrite formation. PMID: 21835341
  19. These data are consistent with a model whereby Sema3A and 3F, in combination with other guidance molecules, contributes to the navigation of DA axons to their final synaptic targets. PMID: 20298787
  20. the expression of Sema3A is increased markedly in basal cells of the newly healed corneal epithelium, and this up-regulation of Sema3A is not associated with cell proliferation. PMID: 20331965
  21. NRP2 is necessary to trigger Sema3A-induced glioma cell repulsion and attraction. PMID: 19855168
  22. Sema3A-induced growth cone collapse of DRG neurons was suppressed by expression of Fes-negative mutants. Fes links Sema3A signals to CRMP-CRAM, and that NP-1 negatively regulates PlexA1 activation by Fes in resting condition. PMID: 12093729
  23. SEMA3 may havea role in modulating the cell fate of axotomized retinal ganglion cells PMID: 12376549
  24. Sema3A regulates the migration of GABAergic neurons in the developing neocortex PMID: 12454988
  25. The expression profile of Sema3A mRNA and the axonal growth-associated protein GAP-43 mRNA during the process of electrically induced epileptogenesis in rats was investigated PMID: 12821384
  26. These results suggest that Sema3A is regulated differently in spinal motoneurons and brain neurons following axonal injury. PMID: 14727128
  27. We investigated the ability of semaphorin3A to selectively reduce NGF-induced sprouting and neuropathic pain in adult rats. Mature sensory afferents maintain their responsiveness to semaphorin3A suggesting it might be used therapeutically PMID: 14749426
  28. The data of this study imply that Sema3A acts as a short-range repellent that regulates the timing of target contact by trigeminal axons. PMID: 14755522
  29. Semaphorin3A mRNA and protein are up-regulated in the scar and in motoneurons from 3 days and upto 3 weeks after injury. PMID: 15094469
  30. association with proteoglycans in the extracellular matrix of neuronal cells plays an important role in the localization of the chemorepulsive guidance cue Sema3A, and that this interaction may enhance its biological activity PMID: 15866045
  31. The chemorepulsion studies are consistent with a role for Sema3A, not Sema3F, in restricting geniculate axons from the epithelium until E17, when axons penetrate the epithelium. PMID: 16217616
  32. novel semaphorin-mediated mechanism of neuroprotection whereby stressed neurons can protect themselves from further damage by activated microglia. PMID: 16467521
  33. Expression of Sema3A by Schwann cells not only suppresses nerve terminal plasticity at specific neuromuscular synapses, but may also contribute to their early and selective loss in the motor neuron disease ALS. PMID: 16677822
  34. We suggest that cell invasion, semaphorin 3A and neuropilin-1 induction, and disruption of the internal organization of the optic nerve contribute to axotomy-induced degenerative processes. PMID: 16906543
  35. Sema3A enhanced axon growth PMID: 18041777
  36. From this study suggested Sema3A may function as a chemoattractive guidance signal for the radial migration of newborn cortical neurons toward upper layers. PMID: 18059265
  37. Semaphorin3A was up-regulated and reached its peak at two time points: 24 hours and 14 days after surgery in retinal detachment model PMID: 18815803
  38. Data highlight an active role for satellite cells as a source of Semaphorin 3A expression triggered by HGF, suggesting that regenerative activity may reside in satellite cells and could play a role during postnatal myogenesis. PMID: 19515904

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Subcellular Location Secreted
Protein Families Semaphorin family
Database Links

KEGG: rno:29751

STRING: 10116.ENSRNOP00000036472

UniGene: Rn.44410


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