Recombinant Saccharomyces cerevisiae Acyl-CoA-binding protein (ACB1) is expressed in yeast and includes an N-terminal 6xHis tag for efficient purification. This full-length protein spans amino acids 1-87 and shows purity greater than 90% as confirmed by SDS-PAGE. Intended for research use only, it appears to be a reliable tool for various biochemical assays, with low endotoxin levels that make it suitable for sensitive applications.
The Acyl-CoA-binding protein (ACB1) from Saccharomyces cerevisiae likely plays a crucial role in intracellular lipid metabolism. It participates in the binding and transport of acyl-CoA esters, supporting key processes in lipid biosynthesis and degradation. Given its fundamental role in lipid homeostasis, ACB1 has become an important focus in research studying metabolic pathways in eukaryotic cells, particularly in yeast models.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Lipid Metabolism Research and Acyl-CoA Binding Studies
This recombinant ACB1 protein can be used to investigate acyl-CoA binding specificity and affinity in yeast lipid metabolism pathways. Researchers may perform in vitro binding assays to characterize how the protein interacts with different acyl-CoA species and determine binding kinetics. The N-terminal 6xHis tag simplifies purification and immobilization for surface plasmon resonance or other biophysical binding studies. Such experiments would contribute to understanding how acyl-CoA-binding proteins function in cellular lipid homeostasis and fatty acid metabolism regulation.
2. Protein-Protein Interaction Studies
Recombinant ACB1 can serve as bait or prey in pull-down assays to identify novel protein interaction partners involved in yeast metabolism. The 6xHis tag allows efficient capture using nickel-affinity resins, permitting co-purification of interacting proteins for mass spectrometry analysis. These studies might reveal new components of lipid metabolic complexes or regulatory networks in Saccharomyces cerevisiae. Interaction mapping experiments like these are valuable for constructing comprehensive protein interaction networks related to cellular metabolism.
3. Antibody Development and Validation
This purified recombinant protein can function as an antigen for generating specific antibodies against yeast ACB1. The high purity (>90%) makes it suitable for immunizing animals or screening hybridoma clones for antibody production. Researchers can use the protein in ELISA-based assays to validate antibody specificity and determine optimal working concentrations. The 6xHis tag also provides tag-specific detection methods to confirm protein expression and localization in experimental systems.
4. Comparative Protein Structure and Function Analysis
Recombinant ACB1 may be used in comparative studies with acyl-CoA-binding proteins from other species to understand evolutionary conservation and functional differences. Researchers can perform structural characterization using techniques such as circular dichroism spectroscopy or NMR to analyze protein folding and stability. Cross-species binding assays might reveal differences in substrate specificity or binding affinity that could reflect evolutionary adaptations. These comparative studies contribute to understanding the functional evolution of lipid-binding proteins across different organisms.
