Recombinant Saccharomyces cerevisiae Cell division control protein 48 (CDC48), partial

1. Identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome. PMID: 28525741
2. Study elucidated the mechanism of Cdc48 from S. cerevisiae with purified components that include Cdc48 itself, its UN cofactor, and model substrates bearing K48-linked polyubiquitin chains. PMID: 28475898
3. Multispanning endoplasmic reticulum membrane proteins, called ERAD-M substrates, are retrotranslocated to the cytosol as full-length intermediates during Endoplasmic Reticulum-associated Degradation, and this study investigated how they maintain substrate solubility. the Cdc48 complex is generally and critically needed for the solubility of retrotranslocated ERAD-M intermediates. PMID: 28077573
4. Data show that Saccharomyces cerevisiae proteins Rqc1, Ltn1, and Cdc48 prevent the aggregation of aberrant proteins that can only appear after C-terminal alanine-threonine tail (CAT-tail) addition by Rqc2. PMID: 27129255
5. Upon DNA damage Wss1/Cdc48/Doa1 ternary complex is recruited to sumoylated targets and catalyzes SUMO chain extension through a newly recognized SUMO ligase activity. PMID: 26349035
6. The tethering of SCF(Dia2) to the replisome progression complex increases the efficiency of ubiquitylation of the Mcm7 subunit of CMG. Correspondingly, loss of tethering is synthetic lethal in combination with a disassembly-defective allele of CDC48. PMID: 26255844
7. conclude that budding yeast Cdc48 functions downstream of the Ub ligases Ltn1 and Ubr1 to release nascent proteins from the ribosome so that they can be degraded by the proteasome PMID: 23358411
8. This suggests that the ATPase activity of Cdc48p is necessary for fusion of the outer membranes of mitochondria. PMID: 24893221
9. Cdc48 segregase is required to break ubiquitylated CMG helicase into its component parts. PMID: 25342810
10. Cdc48-Ubx4 acts on the proteasome and uses the chaperone activity to promote its nuclear distribution, thereby optimizing the proteasome level for efficient degradation of mitotic regulators. PMID: 24225956
11. Cuz1 helps limit the accumulation of ubiquitin conjugates on both the proteasome and Cdc48, suggesting a possible role in the transfer of ubiquitylated substrates from Cdc48 to the proteasome PMID: 24121501
12. The actions of Cdc48p/p97 and the proteasome are tightly coupled during endoplasmic reticulum-associated degradation. PMID: 23536702
13. Study identified over 100 highly interconnected genes that affect stress granules and/or P bodies; additional analyses provided evidence that clearance of these mRNP granules is affected by both autophagy and Cdc48/VCP function, thus identifying a new mechanism for clearing these assemblies from the cell. PMID: 23791177
14. Data suggest that the Cdc48(Shp1) complex controls Glc7 activity by regulating its interaction with Glc8 and possibly further regulatory subunits. PMID: 23418575
15. SUMO-targeted Cdc48 restricts the recombinase Rad51 by counterbalancing the activity of Rad52. PMID: 23624404
16. ubiquitination of the nascent peptide starts on the 80S and continues on the 60S, on which Cdc48 is recruited to escort the substrate for proteasomal degradation. PMID: 23479637
17. Our study describes a critical role for Cdc48/p97 in signal-induced disassembly of the SCF ubiquitin ligase complex for coordinated inhibition of substrate ubiquitylation PMID: 23000173
18. The results suggest that Cdc48p controls mitochondrial morphology by regulating turnover of proteins involved in mitochondrial morphology in a Vms1p-independent manner. PMID: 22580068
19. Data establish Cdc48/p97-Ufd1-Npl4 as a crucial negative regulator of Aurora B early in mitosis of human somatic cells and suggest that the activity of Aurora B on chromosomes needs to be restrained to ensure faithful chromosome segregation. PMID: 21486945
20. Dfm1 forms a complex with the AAA-ATPase Cdc48 in a strain lacking the Cdc48 membrane-recruiting component Ubx2. PMID: 20579315
21. Ubiquitylation of Sec23 is controlled by the Rsp5 ubiquitin ligase. Cdc48 controls the proteasome-mediated degradation of Sec23. PMID: 20846524
22. Data show that the yeast Ubp3-Bre5 deubiquitination complex interacts with both the chaperone-like Cdc48, a major actor of the ubiquitin and proteasome system, and Ufd3, a ubiquitin-binding cofactor of Cdc48. PMID: 20508643
23. Cdc48-Shp1 promotes nuclear accumulation of Glc7 to counteract Ipl1 activity. PMID: 20483956
24. Ssz1 restores endoplasmic reticulum-associated protein degradation in cells expressing defective cdc48-ufd1-npl4 complex by upregulating cdc48. PMID: 20038635
25. The high resolution crystal structure of the p97-PLAA complex was solved showing that the PUL domain forms a 6-mer Armadillo-containing domain. PMID: 19887378
26. Dsk2p, Rad23p and the trimeric Cdc48 complex function together in the delivery of ubiquitinated proteins to the proteasome PMID: 15167887
27. degradation of CFTR requires the ubiquitin protein ligases Der3p/Hrd1p and Doa10p as well as the cytosolic trimeric Cdc48p-Ufd1p-Npl4p complex PMID: 15215312
28. Data suggest that Shp1 and Ubx2 are adaptors for Cdc48-dependent protein degradation through the ubiquitin/proteasome pathway. PMID: 15258615
29. Cdc48 associates with Ufd2, an E4 multiubiquitylation enzyme that adds further ubiquitin moieties to preformed ubiquitin conjugates to promote degradation. PMID: 16427015
30. The PUL and PFU domains of Doa1 promote the formation of a Doa1-Cdc48-ubiquitin ternary complex, potentially allowing for the recruitment of ubiquitinated proteins to Cdc48. PMID: 16428438
31. The Hrd1p ligase complex unites substrate selection in the ER lumen and polyubiquitination in the cytoplasm and links these processes to the release of ER proteins via the Cdc48p complex. PMID: 16619026
32. there is mitochondrial involvement in Cdc48p/VCP-dependent apoptosis PMID: 16822868
33. Dfm1p interacts with Cdc48p through its SHP boxes, and so defines a new motif for interaction with this widely-employed AAA-ATPase. PMID: 17083136
34. One Ufd1-Npl4 heterodimer is shown to interact with one p97 hexamer to form the p97-Ufd1-Npl4 complex. PMID: 17202270
35. Cdc48p functions along with the Npl4p-Ufd1p complex as a polyubiquitin-selective segregase. PMID: 17289586
36. Cdc48 and Rpt4 have cooperative roles in dislocation of endoplasmic reticulum-associated degradation (ERAD) substrates PMID: 18174173
37. The observation that Ubx2 and Ubx4 are not found together in one complex with Cdc48 suggests several distinct steps in modulating the activity and localization of Cdc48 in endoplasmic reticulum PMID: 19359248
38. Data provide direct evidence that the functions of Ufd3 in ubiquitin homeostasis and protein degradation depend on its interaction with the C terminus of Cdc48. PMID: 19805280
39. findings expand the functional targets of Cdc48 to include active transcriptional regulatory complexes in the nucleus. PMID: 19915556

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KEGG: sce:YDL126C

STRING: 4932.YDL126C