Recombinant Saccharomyces cerevisiae DNA repair and recombination protein PIF1 (PIF1), partial

1. Dna2 processes behind the fork long ssDNA flaps generated by Pif1 and replication-dependent strand displacement. PMID: 30446656
2. It has been proposed that Rrm3 and Pif1 promote genome stability at tDNAs by displacing the stable multi-protein transcription complex and by removing R-loops. PMID: 28429714
3. we provide in vivo evidence that Pif1 inhibits telomerase activity at DNA ends regardless of telomere sequence length. PMID: 29052759
4. Multiple Pif1 helicases are required to sequentially disrupt G-quadruplex structure and unwind duplex DNA PMID: 27079238
5. Either Pif1 or Rrm3 redundantly stimulates strand displacement by DNA polymerase delta during lagging-strand synthesis. PMID: 27991904
6. The DNA unwinding activity of Pif1 can be regulated by force. PMID: 27098034
7. Pif1 not only stimulates the strand displacement activity of Pol delta but it also allows efficient replication through the Rap1 block, by removing bound Rap1 in front of the polymerase. PMID: 27001517
8. In budding and fission yeasts, Pif1 family helicases impact both telomerase-mediated and semi-conservative replication of telomeric DNA as well as recombination-mediated telomere lengthening. (Review) PMID: 27233114
9. Using a DNA polymerase coupled assay and FRET (Forster resonance energy transfer)-based helicase assays, in this work, the authors show that a monomer of Saccharomyces cerevisiae Pif1 can unwind double-stranded DNA. PMID: 26908222
10. Pif1 is capable of unwinding RNA:DNA heteroduplexes with moderately greater processivity compared with its duplex DNA:DNA counterparts. PMID: 26733194
11. Rad53-mediated regulation of Rrm3 and Pif1 DNA helicases contributes to prevention of aberrant fork transitions under replication stress. PMID: 26411679
12. The role of Pif1 in break induced replication and telomere lengthening:break-induced replication requires DNA damage-induced phosphorylation of Pif1 and leads to telomere lengthening. PMID: 25329304
13. preferential lengthening of short yeast telomeres is achieved in part by targeting the negative regulator Pif1 to long telomeres PMID: 25906395
14. periodic patrolling activity may keep Pif1 at its site of in vivo action in displacing telomerase, resolving R-loops, and keeping G4 unfolded during replication, recombination and repair PMID: 24843019
15. Pif1 helicase unfolds G-quadruplexes in sequential and repetitive steps. PMID: 25471447
16. Pif1 can anneal DNA in the presence of ATP and Mg(2+). Pif1-mediated annealing also occurs in the presence of single-stranded DNA binding proteins. PMID: 25393406
17. The enhanced telomerase removal efficiency by Pif1 at the longer single-stranded telomeric DNA suggests a way of how Pif1 regulates telomerase activity and maintains telomere length. PMID: 24981509
18. Pif1 stimulates DNA synthesis during break-induced replication and crossover recombination PMID: 24025768
19. Results show that monomers of Pif1 are able to translocate on single-stranded DNA with 5' to 3' directionality. PMID: 23446274
20. The data suggests that G4 structures form in vivo and that they are resolved by Pif1 to prevent replication fork stalling and DNA breakage. PMID: 21620135
21. Pif1p is not only bound to mitochondrial DNA but also to the inner mitochondrial membrane either directly or indirectly via a protein complex. PMID: 20655619
22. Pif1 removes Okazaki fragments initiated by fold-back flaps in vivo PMID: 20959454
23. it is postulated that lack of Pif1 forces aconitase to play its DNA protective role as a nucleoid protein and that this triggers a domino effect on iron homoeostasis resulting in increased zinc tolerance PMID: 20858222
24. Strains lacking Pif1p exhibit an increased rate of formation of petite mutants, an indicator of mtDNA instability, and elevated mtDNA point mutagenesis. PMID: 15907372
25. Pif1p helicase activity limits telomerase action both in vivo and in vitro by displacing active telomerase from DNA ends PMID: 16121131
26. Has a direct role in the prevention or repair of Sgs1-induced DNA damage that accumulates in top3 mutants. PMID: 16816432
27. Pif1p participates in the protection from double-stranded (ds) DNA breaks or alternatively in the repair process of double-stranded DNA breaks in mtDNA. PMID: 17257907
28. Forked RNA-DNA substrates are the favored substrates for Pif1p in vitro. PMID: 17720711
29. Pif1 directs long flaps toward the two-nuclease pathway, requiring Dna2 cleavage for primer removal. PMID: 18689797
30. Pif1p acts as a DNA helicase in mitochondria affects mtDNA replication directly and independently of the DNA helicase Rrm3p PMID: 19277716
31. CEB1 repeats formed stable G-quadruplex (G4) secondary structures, and the Pif1 protein unwinds these structures more efficiently than regular B-DNA. PMID: 19424434
32. Pif1 helicase lengthens some Okazaki fragment flaps necessitating Dna2 nuclease/helicase action in the two-nuclease processing pathway PMID: 19605347

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KEGG: sce:YML061C

STRING: 4932.YML061C