This recombinant Saccharomyces cerevisiae HEM2 is a semi-custom product. There are 5 expression system options: Yeast, E. coli, In Vivo Biotinylation in E. coli, Baculovirus, and Mammalian cell. Your requirements will be given top priority in determining the protein tags. For proteins within 800 aa, risk-free custom service is guaranteed. It means you will not be charged if the protein cannot be delivered.
HEM2, also called Delta-aminolevulinic acid dehydratase (d-ALAD), is a crucial enzyme in the heme biosynthesis pathway. It catalyzes the condensation of two molecules of delta-aminolevulinic acid (d-ALA) to form porphobilinogen (PBG) [1]. It is vital in producing heme essential for various biological processes. Mutations or deficiencies in the delta-aminolevulinic acid dehydratase gene can lead to disorders such as delta-aminolevulinic acid dehydratase-porphyria (ADP), an autosomal recessive disorder affecting heme biosynthesis [2]. Lead exposure can inhibit d-ALAD activity, which causes an increased excretion of δ-ALA in urine [3]. Studies on avian species have shown that even low lead levels exposure can significantly reduce δ-ALAD activity, indicating potential adverse effects on wild populations [4]. Additionally, the activity of this enzyme can be used as a biomarker for lead poisoning and neurotoxic effects in various populations, including urban adolescents and pottery workers [5].
References:
[1] M. Ahamed, S. Verma, A. Kumar, & M. Siddiqui, Delta-aminolevulinic acid dehydratase inhibition and oxidative stress in relation to blood lead among urban adolescents, Human & Experimental Toxicology, vol. 25, no. 9, p. 547-553, 2006. https://doi.org/10.1191/0960327106het657oa
[2] R. Neeleman, E. Beers, E. Friesema, R. Koole-Lesuis, W. Pol, J. Wilsonet al., Clinical remission of delta‐aminolevulinic acid dehydratase deficiency through suppression of erythroid heme synthesis, Hepatology, vol. 70, no. 1, p. 434-436, 2019. https://doi.org/10.1002/hep.30543
[3] N. Patil, S. Kalokhe, S. Patharkar, A. Nerurkar, P. Surve, & U. Shinde, Study of estimation of urinary delta aminolevulinic acid levels as an indicator of lead exposure in pottery workers, Asian Journal of Medicine and Health, p. 58-63, 2020. https://doi.org/10.9734/ajmah/2020/v18i830234
[4] J. Holladay, M. Nisanian, S. Williams, R. Tuckfield, R. Kerr, T. Jarrettet al., Dosing of adult pigeons with as little as one #9 lead pellet caused severe δ-alad depression, suggesting potential adverse effects in wild populations, Ecotoxicology, vol. 21, no. 8, p. 2331-2337, 2012. https://doi.org/10.1007/s10646-012-0989-x
[5] N. Patil, S. Kalokhe, S. Patharkar, A. Nerurkar, P. Surve, & U. Shinde, Study of estimation of urinary delta aminolevulinic acid levels as an indicator of lead exposure in pottery workers, Asian Journal of Medicine and Health, p. 58-63, 2020. https://doi.org/10.9734/ajmah/2020/v18i830234
