Product Details

Purity

>85% (SDS-PAGE)

Target Names

SEC61

Uniprot No.

P32915

Alternative Names

SEC61; YLR378C; L3502.5; Protein transport protein SEC61; Sec61 complex subunit SEC61; Sec61 complex subunit alpha

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Part of the Sec61 complex, which is the major component of a channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). The functional states of the translocon complex include co- and post-translational ER import, cotranslational membrane protein integration and retrograde transport of misfolded proteins out of the ER. In the cotranslational pathway, ribosomes synthesizing presecretory proteins are targeted to the translocon by the cytosolic signal recognition particle (SRP) and its ER-localized receptor. The association of the Sec61 complex with the ribosome is mediated by the 28S rRNA of the large ribosomal subunit. SRP-independent post-translational translocation requires the association of additional factors, such as the Sec62/63 complex and KAR2. In an initial step, the signal sequence seems to bind simultaneously to SEC61 and SEC62. A cycle of assembly and disassembly of Sec62/63 complex from SEC61 may govern the activity of the translocon. SEC61 mediates the association with the ribosome.

Gene References into Functions

Increased endoplasmic reticulum glutathione import triggers H2O2-dependent Bip oxidation through Ero1 reductive activation, which inhibits glutathione import in a negative regulatory loop. PMID: 28918898
These data suggest that the interaction between the proteasome 19S regulatory particle and the Sec61 channel is essential for the export of specific substrates from the endoplasmic reticulum to the cytosol for proteasomal degradation. PMID: 25658429
To map key residues in TM2b and TM7 in yeast Sec61 that modulate lateral gating activity, we performed alanine scanning and in vivo site-directed photocross-linking experiments. PMID: 24753257
Loop 7 of Sec61 is required for initiation of posttranslational soluble protein import into and misfolded soluble protein export from the endoplasmic reticulum, suggesting a key role for L7 in transverse gating of the Sec61 channel. PMID: 24314051
A functional asymmetry exists within the SEC61 translocon caused by the residues forming the central constriction in the translocation pore. PMID: 24191046
are consistent with a Sec61 requirement for peroxisome formation PMID: 22212716
these results demonstrate a novel Sec61 point mutation that clearly implicates the 4th ER luminal loop of this protein in the ERAD process PMID: 23044417
CaM binding to the cytosolic N-terminus of Sec61alpha is involved in limiting Ca(2+)-leakage from the ER in C. familiaris but not S. cerevisiae. PMID: 21558796
Sec61 post-translational translocation is dependent on assembly with Sss1p complexes. PMID: 21355855
Sec61 complexes from yeast S. cerevisiae and C. familiaris both displayed conformable characteristics of a highly dynamic channel in an intrinsically open state. PMID: 20450886
Data suggest that mutations in cytoplasmic loops six (L6) and eight (L8) of sec61 affect different steps in the cotranslational translocation pathway. PMID: 15631991
The results suggest that the plug is unlikely to be important for sealing the translocation pore in yeast but that it plays a role in stabilizing Sec61p during translocon formation. PMID: 16822836
The proteasome interaction with the Sec61 channel in the endoplasmic reticulum was characterized. PMID: 17264153
similar correlation between topology defects and prl phenotype was also seen with previously identified yeast Sec61 mutants PMID: 17893139
The substrate protein forms a disulfide-linked complex to Sec61p, suggesting that at least part of the retrotranslocation process involves Sec61p. PMID: 18573918
analysis of how Sec61p is required for ERAD-L, and interactions of Sec61P and CPY PMID: 18819915
Hrd3p, which is part of the Hrd-Der ubiquitin ligase complex, and other core components of the endoplasmic reticulum-associated degradation machinery physically interact with Sec61p. PMID: 19696741
The beta-subunit of Sec61 protein interacts with ER-resident reticulon protein complexes. PMID: 19857245

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

SecY/SEC61-alpha family

Database Links

KEGG: sce:YLR378C

STRING: 4932.YLR378C

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Code	CSB-YP327181SVG
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Size	Pls inquire
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Code	CSB-EP327181SVG
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Code	CSB-EP327181SVG-B
MSDS	MSDS
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Code	CSB-BP327181SVG
MSDS	MSDS
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Code	CSB-MP327181SVG
MSDS	MSDS
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Source	Mammalian cell
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Recombinant Saccharomyces cerevisiae Protein transport protein SEC61 (SEC61), partial

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