Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase MEC1 (MEC1), partial

1. that proteostasis is a fundamental function of Mec1 and that Mec1 is likely to utilize its checkpoint response network to mediate resistance to proteotoxic stress PMID: 30130531
2. Mec1 initiates checkpoint signaling, promotes DNA replication, and maintains genetic stability through distinct modes of action. PMID: 29899143
3. hese unusual mcm2DENQ phenotypes are markedly similar to those of a special previously-studied allele of the checkpoint sensor kinase ATR/MEC1, suggesting a possible regulatory interplay between Mcm2-7 and ATR during unchallenged growth. PMID: 27556397
4. Mec1 is highly activated during normal DNA replication. PMID: 25752575
5. Mms21 was phosophorylated during S-phase in a manner dependent on the DNA damage kinase Mec1. S260 S261 phosphorylation may positively regulate the SUMO ligase activity of Mms21 and promote genomic stability. PMID: 25659338
6. RNA-processing proteins regulate Mec1/ATR activation by promoting generation of RPA-coated ssDNA PMID: 25527408
7. the SWI/SNF chromatin remodeling complex is capable of regulating Mec1 kinase activity PMID: 25792597
8. identified a Mec1/ATR- and Dbf4-dependent replication checkpoint in budding yeast that prevents the earliest stage of recombination PMID: 24137535
9. Our results suggest that Ddc2 plays a critical role in Mec1 activation as well as Mec1 localization at sites of DNA damage. PMID: 24586187
10. C-termunal FATC domain of Mec1 is required for Mec1 folding, stability and nuclear localization. PMID: 23934994
11. Mec1 regulates the generation of ssDNA at DSBs, and this control is important to coordinate Mec1 and Tel1 signaling activities at these breaks. PMID: 24357557
12. Antagonistic regulation of DSB formation by Mec1 and Tel1 creates a regulatory mechanism, where the absolute frequency of DSBs is maintained at a level optimal for genetic exchange and efficient chromosome segregation. PMID: 23902647
13. Mec1 may facilitate DNA replication fork progression during replication stress by increasing chromatin accessibility around replication forks. PMID: 23307868
14. in contrast to Tel1p, Mec1p associates with short, functionally compromised telomeres. PMID: 22289863
15. Mutant analysis shows that enhanced double-strand-break mobility requires Rad51, the ATPase activity of Rad54, the ATR homologue Mec1 and the DNA-damage-response mediator Rad9 PMID: 22484486
16. DNA damage-induced sumoylation does not require Mec1 checkpoint signaling, and the presence of both enables optimal DNA damage resistance PMID: 22285753
17. Epistatic analysis demonstrated that the rad31 mutation is an allele of the MEC1 gene, which allows further designation of the rad31 mutation as mec1-212. PMID: 21786666
18. Dpb11 and the 9-1-1 complex independently promote Mec1 activation PMID: 21436894
19. Rrm3Delta and high concentrations of hydroxyurea suppressed replication slow zone expression in mec1-4ts cells. PMID: 21172804
20. Mec1 mutations are associated with increased rates of spontaneous loss of heterozygosity leading to genome instability. PMID: 20410660
21. When cells equipped with short telomeres, recruitments of homologous recombination proteins, Rad51 and Rad52, were reduced at an HO-endonuclease-catalyzed double-strand break (DSB), while their associations were increased at chromosome ends. PMID: 20011546
22. Cdc5 override the Mec1/ATR checkpoint by acting at different steps of the signaling pathway. PMID: 20098491
23. G1 phase checkpoint activation of Mec1 is achieved by the Ddc1 subunit of 9-1-1. PMID: 20005839
24. These findings establish a direct role for Mec1 kinase in transcription-coupled repair, at least partly via phosphorylation of Rad26, the main transcription-repair coupling factor. PMID: 19901073
25. identify Regulator of Ty1 Transposition as an important new S-phase-specific target of Mec1p PMID: 14988729
26. model in which the Mre11 complex and Exo1 cooperate in generating long ssDNA tracts and thereby facilitate Mec1 association with sites of DNA damage or replication block PMID: 15509802
27. Multiple DNA damage checkpoint kinase Mec1 regulates the Asf1-Rad53 interaction and therefore affects the activity of the Asf1/Hir complex in vivo. PMID: 16020781
28. a new relationship between Cu, Zn superoxide dismutase, Copper chaperone for superoxide dismutase Sod1p, and the MEC1-mediated checkpoint response to replication arrest and DNA damage in Saccharomyces cerevisiae PMID: 16287844
29. deletion of YKU70 or YKU80 suppresses mec1Delta, but not rad53Delta, lethality PMID: 16287875
30. Activation of the checkpoint kinase Rad53 by the phosphatidyl inositol kinase-like kinase Mec1. PMID: 16365046
31. Mec1 has a role in the localization of the Polzeta-Rev1 complex to DNA lesions. PMID: 16546083
32. Here we have studied the role of the clamp in regulating Mec1, and we delineate how the signal generated by DNA lesions is transduced to the Rad53 effector kinase. PMID: 17189191
33. Control of telomeric recombination by Mec1. PMID: 17202155
34. The DNA damage checkpoint clamp Rad17-Mec3-Ddc1 (human 9-1-1) is loaded onto gapped DNA resulting from the partial repair of DNA damage, and the Ddc1 subunit of this complex activates the checkpoint protein kinase Mec1. PMID: 17495536
35. review current data on meiotic defects associated with loss of Mec1 functions and discuss the possibility that Mec1 may participate as a fundamentally positive player in coordinating and promoting basic meiotic chromosomal processes during normal meiosis PMID: 17674144
36. Dpb11 and yeast 9-1-1 independently activate Mec1, but substantial synergism in activation was observed when both activators were present PMID: 18922789
37. Both X ray and UV exposure increased translocation frequencies in mec1-21, but the majority of the UV-associated products were non-reciprocal translocations. PMID: 18929581
38. a single amino acid substitution in the C terminus, which could lead to an allosteric change in Chk1, allows it to bypass the requirement of the conserved ATR(Mec1) phosphorylation sites for checkpoint function. PMID: 18984588
39. Tel2 can specifically and differentially regulate the function of individual PIKKs. PMID: 19029808
40. These results indicate that Cdc28-Mec1 activity inhibits the recruitment of Rad52 to both sites of DNA damage and stalled replication forks during the intra-S-phase checkpoint. PMID: 19262568
41. The mode of Mec1/ATR activation by S. cerevisiae 9-1-1 is analogous to activation by S. cerevisiae Dpb11 or by vertebrate TopBP1: activation is mediated by the intrinsically disordered C-terminal tail of each activator. PMID: 19464966
42. Data suggest that in the absence of telomerase, a very short telomere is first maintained in a pre-signalling state by a RAD52-MMS1-dependent pathway and then switches to a signalling state leading to senescence through a Mec1p-dependent checkpoint. PMID: 19597486

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KEGG: sce:YBR136W

STRING: 4932.YBR136W