Recombinant Saccharomyces cerevisiae Weak acid resistance protein 1(WAR1) ,partial

Code CSB-YP312724SVG
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Source Yeast
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Code CSB-EP312724SVG
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Source E.coli
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Code CSB-EP312724SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP312724SVG
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Source Baculovirus
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Code CSB-MP312724SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names WAR1
Uniprot No. Q03631
Alternative Names
WAR1; YML076C; Weak acid resistance protein 1
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
transcription factor which binds to a weak acid response element (WARE) to mediate stress induction of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively.
Gene References into Functions
  1. Activity of the War1 is lost with alanine mutation of two putative phosphorylation sites in the activation domain. PMID: 22113732
  2. Data suggest that phosphorylation might play a role in stabilizing War1 activity on the promoter elements, hence changing the dynamics and kinetics of the stress response. PMID: 20726777
  3. Phosphorylation of War1p, its activation and DNA binding are tightly linked processes that are essential for adaptation to weak-acid stress. PMID: 17509074
  4. War1 activation is accompanied by conformational changes enhancing promoter association, thus initiating the adaptation process PMID: 18621731
Subcellular Location Nucleus.
Database Links

KEGG: sce:YML076C

STRING: 4932.YML076C

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Address
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
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