Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
ompD; nmpC; STM1572; Outer membrane porin protein OmpD
Species
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Expression Region
22-362aa
Target Protein Sequence
AEVYNKDGNKLDLYGKVHAQHYFSDDNGSDGDKTYARLGFKGETQINDQLTGFGQWEYEFKGNRTESQGADKDKTRLAFAGLKFADYGSFDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNTDFFGLVEGLNFAAQYQGKNDRDGAYESNGDGFGLSATYEYEGFGVGAAYAKSDRTNNQVKAASNLNAAGKNAEVWAAGLKYDANNIYLATTYSETLNMTTFGEDAAGDAFIANKTQNFEAVAQYQFDFGLRPSIAYLKSKGKNLGTYGDQDLVEYIDVGATYYFNKNMSTFVDYKINLLDDSDFTKAAKVSTDNIVAVGLNYQF
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Salmonella typhimurium ompD contains amino acids 22-362. The theoretical molecular weight of the ompD protein is 53.6 kDa. The ompD protein was expressed in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of ompD, which enables a simple process of detecting and purifying the ompD recombinant protein in the following steps.
The outer membrane porin protein (OmpD) in Salmonella typhimurium is a key component of the bacterial outer membrane, contributing to the selective permeability and integrity of the cell envelope. OmpD functions as a porin, forming channels in the outer membrane that allow the passive diffusion of small hydrophilic molecules, including ions and nutrients, across the bacterial membrane. OmpD plays a crucial role in the uptake of essential nutrients from the external environment, such as sugars and amino acids, facilitating the bacterium's survival and growth. Additionally, OmpD is involved in the response to environmental stresses, including exposure to host immune defenses and antimicrobial agents. The protein contributes to the adaptability of Salmonella typhimurium in diverse host environments and is often implicated in the bacterial pathogenesis process. Understanding the structure and function of OmpD provides insights into the mechanisms of bacterial adaptation and virulence.