Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

phoQ

Uniprot No.

P0DM80

Research Area

Others

Species

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Source

E.coli

Expression Region

215-487aa

Target Protein Sequence

WWSLRPIEALAREVRELEDHHREMLNPETTRELTSLVRNLNQLLKSERERYNKYRTTLTDLTHSLKTPLAVLQSTLRSLRNEKMSVSKAEPVMLEQISRISQQIGYYLHRASMRGSGVLLSRELHPVAPLLDNLISALNKVYQRKGVNISMDISPEISFVGEQNDFVEVMGNVLDNACKYCLEFVEISARQTDDHLHIFVEDDGPGIPHSKRSLVFDRGQRADTLRPGQGVGLAVAREITEQYAGQIIASDSLLGGARMEVVFGRQHPTQKEE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

38.5 kDa

Protein Length

partial

Tag Info

N-terminal 10xhis-tagged and C-terminal myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Member of the two-component regulatory system PhoP/PhoQ which regulates the expression of genes involved in virulence, adaptation to acidic and low Mg(2+) environments and resistance to host defense antimicrobial peptides. Essential for intramacrophage survival of S.typhimurium. In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), acts as a protein phosphatase that dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Essential for transcription of spiC inside macrophages by controlling the expression of the two-component regulatory system SsrB/SpiR (SsrA) and Pir at transcriptional and post-transcriptional levels respectively. Promotes expression of the two-component regulatory system PmrA/PmrB via activation of pmrD gene. Is required to attenuate bacterial growth within fibroblast cells and to enhance bacterial resistance to bile in intestinal cells. Negatively regulates prgH, which is required for invasion of epithelial cells. Involved in acid tolerance.

Gene References into Functions

These data suggest that when Salmonella experiences mildly acidic pH, the virulence regulator PhoP activation requires the sensor protein PhoQ to detect pH and the Salmonella-specific protein UgtL to amplify the PhoQ response. PMID: 28851823
Salmonella enterica Typhimurium, bearing the acidic pH and divalent cation unresponsive phoQ(W104C-A128C) allele is virulent in mice, indicating that acidic pH and divalent cation sensing by PhoQ are dispensable for virulence. PMID: 26002083
Changes in the periplasmic domain of PhoQ lead to conformational movements in the HAMP domain helices which disrupt interaction between the HAMP and the catalytic domains, thus promoting increased kinase activity. PMID: 26015499
study describes a mechanism whereby the transcriptional activator PhoP elicits expression of dissimilar gene sets when its cognate sensor PhoQ is activated by different signals in the periplasm PMID: 24256574
These data demonstrated that Salmonella Typhimurium PhoQ can sense cationic antimicrobial peptides and CRAMP serves as a putative direct PhoPQ activation signal in the mouse intestine. PMID: 22919691
PhoP/Q regulon enables S. Typhimurium to adapt to intramacrophage stresses other than phagolysosomal fusion. PMID: 21511762
RstB-induction increases the levels of phoP mRNA as well as PhoP protein, while lack of the phoPQ genes abolishes RstB-promoted transcription of the PhoP-regulated genes PMID: 20811812
Enzymatic activity of Salmonella typhimurium PhoQ is directly activated by [host] antimicrobial peptides. Our findings reveal a mechanism by which bacteria sense small innate immune molecules to initiate a transcriptional program that promotes virulence PMID: 16096064
induction of PhoP/PhoQ system results in initial surge of PhoP phosphorylation, occupancy of target promoters by PhoP & transcription of PhoP-activated genes; surge by PhoP's positive feedback loop is necessary to jump-start virulence program PMID: 17158330
analysis of signal-dependent regulation in the PhoP/PhoQ system PMID: 18434315
Mutational analysis and NMR spectroscopy of the periplasmic domains of Salmonella typhimurium PhoQ and Pseudomonas aeruginosa PhoQ indicate distinct mechanisms of binding divalent cation. PMID: 18532985
SlyA has a role as a mediator in signal transduction from the PhoP/PhoQ system to other bacterial two-component systems in Salmonella PMID: 18678876
Data report that the PhoP/PhoQ system regulates different Salmonella genes depending on whether the inducing signal is acidic pH or low Mg(2+). PMID: 19801407

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Database Links

KEGG: stm:STM1230

STRING: 99287.STM1230

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Code	CSB-EP318475SXB
MSDS	MSDS
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Salmonella typhimurium Virulence sensor histidine kinase PhoQ (phoQ), partial

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