IdeZ is a protease that has been identified as a homolog of IdeS, which is an immunoglobulin-degrading enzyme found in Streptococcus pyogenes [1]. IdeZ shares 99% amino acid sequence identity with IdeE and is more closely related to the Mac-2 protein of S. pyogenes [1]. It has been reported that IdeZ has the same specificity as IdeS but exhibits improved activity towards certain subclasses of immunoglobulin G (IgG) and has been utilized for the characterization of Fc fusion proteins [2]. Furthermore, IdeZ has been implicated in gene therapy, particularly in the context of adeno-associated virus (AAV) gene therapy, where it has shown potential similar to IdeS [3]. Additionally, IdeZ has been used in research to release the Fab portion of antibodies by treating them with IdeZ protease [4]. Moreover, IdeZ has been employed in the production of subunit liquid chromatography-mass spectrometry analysis by coupling with enzymes to produce specific subunits [5].
References:
[1] U. Pawel-Rammingen, "Streptococcal ides and its impact on immune response and inflammation", Journal of Innate Immunity, vol. 4, no. 2, p. 132-140, 2012. https://doi.org/10.1159/000332940
[2] R. O’Flaherty, I. Trbojević‐Akmačić, G. Greville, P. Rudd, & G. Lauc, "The sweet spot for biologics: recent advances in characterization of biotherapeutic glycoproteins", Expert Review of Proteomics, vol. 15, no. 1, p. 13-29, 2017. https://doi.org/10.1080/14789450.2018.1404907
[3] D. Gross, N. Tedesco, C. Leborgne, & G. Ronzitti, "Overcoming the challenges imposed by humoral immunity to aav vectors to achieve safe and efficient gene transfer in seropositive patients", Frontiers in Immunology, vol. 13, 2022. https://doi.org/10.3389/fimmu.2022.857276
[4] J. Earnest, K. Basore, V. Roy, A. Bailey, D. Wang, G. Alteret al., "Neutralizing antibodies against mayaro virus require fc effector functions for protective activity", The Journal of Experimental Medicine, vol. 216, no. 10, p. 2282-2301, 2019. https://doi.org/10.1084/jem.20190736
[5] T. Morgan, C. Jakes, H. Brouwer, S. Millán-Martín, J. Chervet, K. Cooket al., "Inline electrochemical reduction of nistmab for middle-up subunit liquid chromatography-mass spectrometry analysis", The Analyst, vol. 146, no. 21, p. 6547-6555, 2021. https://doi.org/10.1039/d1an01184g
