Product Details

Purity

>85% (SDS-PAGE)

Target Names

elt-2

Uniprot No.

Q10655

Alternative Names

elt-2; C33D3.1Transcription factor elt-2

Species

Caenorhabditis elegans

Expression Region

1-433

Target Protein Sequence

MDNNYNDNVN GWAEMEPSQP MGGLRLPTQN MDPPEQNNES QLSELPRMKI DNDYASPIER QSVITSGTNN YEPKVETVTS FFHTGIDYSN FGMLDQTTMQ PFYPLYSGIP VNTLGTFSGY TNSIYDKPSL YDPSIPTINI PSTYPTVAPT YECVKCSQSC GAGMKAVNGG MMCVNCSTPK TTYSPPVAYS TSLGQPPILE IPSEQPTAKI AKQSSKKSSS SNRGSNGSAS RRQGLVCSNC NGTNTTLWRR NAEGDPVCNA CGLYFKLHHI PRPTSMKKEG ALQTRKRKSK SGDSSTPSTS RARERKFERA SSSTEKAQRS SNRRAGSAKA DRELSTAAVA AATATYVSHA DLYPVSSAAV TLPDQTYSNY YQWNTAATAG LMMVPNDQNY VYAATNYQTG LRPADNIQVH VMPVQDDETK AAARDLEAVD GDS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcriptional activator that binds to the consensus sequence 5'-[AT]GATA[AG]-3'. Predominantly directs the transcription of intestinal genes such as ges-1, cpr-6, pho-1, ftn-1 and lev-11, and itself. Required for gut-specific differentiation, specifically acting with the GATA region-binding transcription factor elt-7 to control normal gene expression and promote normal formation of the intestine. Regulates intestinal gene expression in response to hypoxia to promote longevity. Regulates tissue specific gene expression at basal levels and in response to bacterial infection in the intestine to control innate immununity. Plays a role in the induction of metal-responsive genes, activating gene expression from zinc-activated promoters and iron-dependent promoters and enhancers. May regulate the expression of genes that control sensitivity to oxidative stress, in a mab-3-dependent manner, and osmotic stress, in conjunction with the GATA region-binding transcription factor elt-3. May play a role in sphingolipid signaling by regulating the expression of the sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase. May act with the Notch signaling pathway to promote endodermal gene expression. Has a protective role in response to infection by Gram-negative bacteria such as S.enterica, E.coli, P.aeruginosa and B.pseudomallei, Gram-positive bacterium E.faecalis and fungal pathogen C.neoformans. An association with the 26S proteasome regulatory subunit rpt-6, in part, controls gene expression in response to infection by P.aeruginosa. Regulates gene expression during the recovery phase following a bacterial infection. May act with p38-activated transcription factors to control p38 gene induction in response to bacterial infection. Controls lysosome formation in the intestine by controlling lysosomal gene expression.

Gene References into Functions

gene loss leads to modest increases in the level of ELT-2 protein in the early endoderm although ELT-2 levels do not strictly correlate with rescue. PMID: 29593072
Genes expressed only in the intestine showed 3 distinguishable classes of response to different mutant backgrounds. One class of genes responded as if ELT-2 is the major transcriptional activator and ELT-7 provides variable compensatory input. Appropriately expressed ELT-2 is able to replace all other core GATA factors in the C. elegans endoderm. PMID: 29360433
The authors determined that the GATA transcription factor ELT-2 and the p38 MAP kinase PMK-1 are necessary for animals to successfully recover from an acute Pseudomonas aeruginosa infection. PMID: 27600703
Overexpression of elt-2 extends lifespan and slows the rate of gene expression changes that occur during normal aging. Thus, our results identify the developmental regulator ELT-2 as a major driver of normal aging in C. elegans PMID: 27070429
GATA motifs played largely subtle roles in modulating postembryonic levels of elt-2 PMID: 26896592
ELT-2 is capable of specifying the entire C. elegans endoderm. PMID: 26700680
These results suggest that ELT-2 functions as a tissue-specific master regulator controlling the contribution of the p38 MAPK pathway to innate immune responses. PMID: 26016853
we found that recovery from an acute bacterial infection is dependent on ELT-2 activity. PMID: 25340560
An unexpected feature of the transcriptional response to Burkholderia pseudomallei was a progressive increase in the proportion of down-regulated genes, of which ELT-2 transcriptional targets were significantly enriched. PMID: 23980181
Intestinal distension is accelerated in elt-2 RNAi nematodes, and is observed in colonization but not toxin-based Pseudomonas infection. PMID: 21168435
the intestinal GATA transcription factor ELT-2 is required for both immunity to Salmonella enterica and expression of a C-type lectin gene, clec-67, which is expressed in the intestinal cells and is a good marker of S. enterica infection PMID: 17183709
Results identify a conserved function for endodermal GATA transcription factors GATA6 and ELT-2 in regulating local epithelial innate immune responses. PMID: 16968778
A simple model is proposed in which the ELT-2 GATA factor directly participates in the transcription of all intestine-specific/intestine-enriched genes, from the early embryo through to the dying adult. PMID: 17113066
Results suggest that ELT-2 plays a central role in most aspects of C. elegans intestinal physiology. PMID: 19111532

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in the intestine.

Database Links

KEGG: cel:CELE_C33D3.1

STRING: 6239.C33D3.1

UniGene: Cel.6404

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Recombinant Transcription factor elt-2 (elt-2)

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