Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

lcrV

Uniprot No.

P0C7U7

Research Area

Others

Alternative Names

(Low calcium response locus protein V)

Species

Yersinia pestis

Source

E.coli

Expression Region

1-326AA

Target Protein Sequence

MIRAYEQNPQHFIEDLEKVRVEQLTGHGSSVLEELVQLVKDKNIDISIKYDPRKDSEVFANRVITDDIELLKKILAYFLPEDAILKGGHYDNQLQNGIKRVKEFLESSPNTQWELRAFMAVMHFSLTADRIDDDILKVIVDSMNHHGDARSKLREELAELTAELKIYSVIQAEINKHLSSSGTINIHDKSINLMDKNLYGYTDEEIFKASAEYKILEKMPQTTIQVDGSEKKIVSIKDFLGSENKRTGALGNLKNSYSYNKDNNELSHFATTCSDKSRPLNDLVSQKTTQLSDITSRFNSAIEALNRFIQKYDSVMQRLLDDTSGK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

53.3 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Yersinia pestis lcrV was expressed with the amino acid range of 1-326. The calculated molecular weight for this lcrV protein is 53.3 kDa. Expression of this lcrV protein is conducted in e.coli. The lcrV coding gene included the N-terminal 10xHis-SUMO tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant lcrV protein in following stages of expression and purification.

The Yersinia pestis virulence-associated V antigen (lcrV) is a key protein contributing to the pathogenicity of Yersinia pestis, the causative agent of plague. LcrV is a component of the type III secretion system (T3SS), a molecular apparatus used by Y. pestis to inject effector proteins directly into host cells. LcrV plays a pivotal role in the translocation of these effectors, modulating host immune responses and aiding in the establishment of infection. It is involved in the formation of the injectisome, a needle-like structure through which Yersinia delivers virulence factors into host cells. Understanding the function of LcrV is crucial for developing strategies to counteract Y. pestis infections and potentially mitigate the impact of plague outbreaks.

Target Background

Function

Possibly involved in calcium regulation of YOP expression, which includes the export process.

Gene References into Functions

findings show that LcrV, the cap protein of bacterial type III secretion needles, is modified by host glutathione and that this modification contributes to the high virulence of Y. pestis in mouse and rat models for bubonic plague; these data suggest that Y. pestis exploits glutathione in host tissues to activate a virulence strategy, thereby accelerating plague pathogenesis PMID: 28512097
The crystal structure of LcrV is reported here without the triple mutation and with a different orientation. PMID: 23695558
Our data indicate that the most abundant molecular species of Y. pestis LcrV do not efficiently activate TLR2-signaling and that TLR2-mediated immunomodulation is unlikely to play a significant role in plague. PMID: 17438030

Subcellular Location

Secreted.

Database Links

KEGG: ype:YPCD1.31c

Code	CSB-EP315209YAS
Abbreviation	Recombinant Yersinia pestis lcrV protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Yersinia pestis Virulence-associated V antigen (lcrV)

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