CD97 Antibody

Datasheet

Code CSB-RA004972A0HU
Size US$350
Uniprot No. P48960
Abbreviation CD97 antigen
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-RA004972A0HU at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen A synthesized peptide
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:50-1:500, IF:1:30-1:200
Relevance Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias CD97 antigen, Leukocyte antigen CD97, CD97, CD97 antigen subunit alpha, CD97 antigen subunit beta, CD97
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Neuroscience
Gene Names CD97
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Function Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity).
Subcellular Location Cell membrane, Multi-pass membrane protein, SUBCELLULAR LOCATION: CD97 antigen subunit alpha: Secreted, extracellular space
Protein Families G-protein coupled receptor 2 family, LN-TM7 subfamily
Tissue Specificity Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and p
Database Links

HGNC: 1711

OMIM: 601211

KEGG: hsa:976

STRING: 9606.ENSP00000242786

UniGene: Hs.466039

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