Product Details

Uniprot No.

P48960

Target Names

CD97

Alternative Names

ADGRE5; CD97; Adhesion G protein-coupled receptor E5; Leukocyte antigen CD97; CD antigen CD97

Species Reactivity

Human

Immunogen

A synthesized peptide

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3A7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:500
IF	1:30-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CD97 is a member of the adhesion G protein-coupled receptor family that plays important roles in cell adhesion, migration, and immune cell regulation. Expressed on leukocytes and various other cell types, CD97 participates in cellular interactions critical to immune responses and has garnered significant attention in neuroscience research for its involvement in neuronal signaling and brain tissue organization.

This recombinant monoclonal antibody, clone 3A7, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically to the last, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format provides excellent signal-to-noise characteristics, while affinity chromatography purification delivers a clean reagent ready for demanding applications.

Validation studies demonstrate robust performance across multiple experimental platforms. Immunohistochemistry testing in paraffin-embedded human lymph node and spleen tissues at 1:100 dilution reveals clear staining patterns consistent with CD97's known expression in lymphoid compartments, using citrate buffer antigen retrieval on a Leica Bond system. For immunofluorescence applications, the antibody successfully detects CD97 in HeLa cells at 1:87.5 dilution, producing distinct signal when paired with Alexa Fluor 488-conjugated secondary antibodies. The antibody is also validated for ELISA, providing flexibility across detection methodologies.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this antibody serves researchers investigating immune cell biology, adhesion receptor signaling, and neuroscience applications where understanding CD97 function is essential to advancing their work.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA004972A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-RA004972A0HU at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Have Questions?	Leave a Message or Start an on-line Chat

CD97 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background