DDX20 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA209536A0HU
Size	US$210
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Image	IHC image of CSB-RA209536A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA209536A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA209536A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA209536A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9UHI6

Target Names

DDX20

Alternative Names

Probable ATP-dependent RNA helicase DDX20 (EC 3.6.4.13) (Component of gems 3) (DEAD box protein 20) (DEAD box protein DP 103) (Gemin-3), DDX20, DP103 GEMIN3

Species Reactivity

Human

Immunogen

A synthesized peptide from human DDX20 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17C8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DDX20, also known as Gemin-3, functions as an ATP-dependent RNA helicase that plays essential roles in small nuclear ribonucleoprotein biogenesis and RNA metabolism. As a core component of the survival of motor neurons complex, DDX20 participates in the assembly of spliceosomal snRNPs, making it a significant target for researchers investigating RNA processing pathways, neurodegenerative conditions, and cellular stress responses.

This recombinant monoclonal antibody, clone 17C8, offers the reproducibility and consistency that demanding research applications require. Developed using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The rabbit IgG format, raised against a synthetic peptide from human DDX20, has been affinity-purified to ensure high specificity for your target.

Validation studies demonstrate robust performance across multiple experimental platforms. Immunohistochemistry analysis in paraffin-embedded human colorectal cancer tissue and testis tissue reveals clear, specific staining patterns at 1:100 dilution using citrate buffer antigen retrieval. For cellular localization studies, immunofluorescence microscopy in HeLa cells shows distinct signal when counterstained with DAPI, enabling visualization of DDX20 distribution within subcellular compartments. Flow cytometry validation in HeLa cells confirms the antibody's utility for quantitative single-cell analysis, with clear separation between specific staining and isotype control populations.

The versatility across immunohistochemistry, immunofluorescence, and flow cytometry applications provides researchers with flexibility to address diverse experimental questions using a single, reliable reagent. This antibody serves investigators studying SMN complex biology, spliceosome assembly, RNA helicase function, and their connections to motor neuron diseases and cancer biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).

Gene References into Functions

The findings show that DDX20 may promote the progression of prostate cancer through the NF-kappaB pathway. PMID: 27121695
In stage-adjusted analysis, the nominal associations of DDX20-rs197412, PTGS2-rs5275, and HSPA5-rs391957 with DFS were detected. However, after FDR correction none of these polymorphisms remained significantly associated with the survival outcomes PMID: 26064972
DP103 defines metastatic potential of human breast cancers. PMID: 25083991
Impairment of miRNA-140 function due to a deficiency of DDX20, a miRNA machinery component, could lead to hepatocarcinogenesis PMID: 22898998
Gemin3 binds with p53, forming a complex, and plays an anti-apoptotic role by repressing the p53 expression in Saos-2 cells. PMID: 22335944
While DDX20 normally suppresses NF-kappaB activity by regulating NF-kappaB-suppressing miRNA-140 function, this suppressive effect was lost in DDX20-deficient cells. PMID: 22445758
we report that EBNA3C directly interacts with Gemin3 through its C-terminal domains PMID: 22174681
NANOS1-PUMILIO2 complex, together with GEMIN3 and small noncoding RNAs, possibly regulate mRNA translation within the chromatoid body of the human germ cells. PMID: 21800163
Data show that the two motor neuropathy-associated mutant HspB8 forms have abnormally increased binding to Ddx20. PMID: 20157854
utilised a targeted screen to identify mRNA associated with SMN, Gemin2 and Gemin3 in the cytoplasm of a human neuroblastoma cell line, SHSY5Y PMID: 20620147
Gemin3 and Gemin4 function in Sm core assembly and correlate the activity of this pathway with Spinal Muscular Atrophy. PMID: 16301532

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Subcellular Location

Cytoplasm. Nucleus, gem.

Protein Families

DEAD box helicase family, DDX20 subfamily

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 2743

OMIM: 606168

KEGG: hsa:11218

STRING: 9606.ENSP00000358716

UniGene: Hs.591405

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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