Product Details

Uniprot No.

Target Names

ENPP3

Alternative Names

Alkaline phosphodiesterase I antibody; ARHR2 antibody; B10 antibody; CD203c antibody; CD203c antigen antibody; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody; E NPP 3 antibody; E-NPP 3 antibody; Ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 antibody; ENPP3 antibody; ENPP3_HUMAN antibody; gp130RB13 6 antibody; gp130RB136 antibody; M6S1 antibody; NPP1 antibody; NPP3 antibody; NPPase antibody; NPPS antibody; Nucleotide pyrophosphatase antibody; PC 1 antibody; PC-1 antibody; PCA1 antibody; PD Ibeta antibody; PD-Ibeta antibody; PDNP1 antibody; PDNP3 antibody; Phosphodiesterase I beta antibody; Phosphodiesterase I/nucleotide pyrophosphatase 3 antibody; phosphodiesterase i/nucleotide pyrophosphatase beta antibody

Species Reactivity

Human, Macaca fascicularis

Immunogen

Recombinant Human ENPP3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

3B2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA,FC

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The ENPP3 monoclonal antibody was generated using the recombinant human SLC39A6 protein as an immunogen. The cDNA of the ENPP3 monoclonal antibody was sequenced to obtain the antibody gene, which was cloned into a plasmid vector. The plasmid vector was then transfected into host cells using a suitable transfection method. The resulting ENPP3 recombinant monoclonal antibody underwent purification through affinity chromatography and was subsequently tested for specificity through ELISA. The antibody demonstrated binding capabilities to the recombinant human ENPP3 (CSB-MP4278MOV) with an EC₅₀ range of 3.313-4.724 ng/mL. It can react with human and macaca fascicularis ENPP3 proteins.

ENPP3 is a transmembrane glycoprotein that plays a role in the regulation of cell growth, differentiation, and mineralization. It is involved in the hydrolysis of extracellular nucleotides to generate inorganic phosphate and nucleoside diphosphates, which are important for the regulation of purinergic signaling pathways. ENPP3 is expressed in various tissues, including bone, kidney, and liver, and its dysregulation has been linked to a number of diseases, including calcification disorders, cancer, and inflammation.

Target Background

Function

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Has also alkaline phosphodiesterase activity.

Gene References into Functions

We showed the ability of the immunological marker CD203c to predict the clinical efficacy of sublingual immunotherapy on rhinitis subjects allergic to Parietaria. PMID: 29702283
Basophil CD203c surface expression reliably discriminated cystic fibrosis with allergic bronchopulmonary aspergillosis from cystic fibrosis with Aspergillus colonization and cystic fibrosis over time. PMID: 26585435
Expression of CD203c on basophils as a marker of immunoglobulin E-mediated (L)-asparaginase allergy. PMID: 23581640
Anaphylactic transfusion reaction in homozygous haptoglobin deficiency detected by CD203c expression on basophils. PMID: 24497482
ENPP3 is a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) PMID: 23960081
The early signaling requirements for the CD11b/CD203c compartment expression and CD63 degranulation provide support for the hypothesis that CD11b and CD203c reside in a similar compartment. PMID: 22722613
Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen PMID: 20975283
Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission PMID: 20159259
Influence of hyperosmotic conditions on basophil CD203c upregulation in patients with food-dependent exercise-induced anaphylaxis. PMID: 20047266
Data show that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils. PMID: 19418203
E-NPP3 is associated with carcinogenesis of human colon cancer and that serum E-NPP3 might be a tumor marker of colon carcinoma PMID: 14533006
E-NPP3 is involved in the infiltration of neoplastic bile duct carcinoma PMID: 15072822
Our data demonstrate that leptin promotes platelet activation, provides a mechanistic basis for the prothrombotic effect of this hormone, and identifies a potentially novel therapeutic avenue to limit obesity-associated cardiovascular disease. PMID: 15886225
In conclusion, using well-defined experimental conditions, the measurement of CD203c up-regulation on basophils in response to specific allergens is as reliable as CD63-BAT for the in vitro diagnosis of patients with IgE-mediated allergy. PMID: 17275019

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Subcellular Location

Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Secreted.

Protein Families

Nucleotide pyrophosphatase/phosphodiesterase family

Tissue Specificity

Detected on bile ducts in liver, and in blood serum (at protein level). Detected in prostate and uterus. Detected on basophils, but not neutrophils.

Database Links

HGNC: 3358

OMIM: 602182

KEGG: hsa:5169

STRING: 9606.ENSP00000350265

UniGene: Hs.486489

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Check Code：

Code	CSB-RA007681MA1HU
Size	US$210
Image	The Binding Activity of Macaca fascicularis ENPP3 with Anti-ENPP3 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis ENPP3 (CSB-MP4278MOV) at 2 μg/mL can bind Anti-ENPP3 recombinant antibody, the EC₅₀ is 3.313-4.724 ng/mL. The Binding Activity of Huamn ENPP3 with Anti- ENPP3 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3(CSB-MP007681HU) at 2 μg/mL can bind Anti-ENPP3 recombinant antibody, the EC₅₀ is 2.151-2.492 ng/mL Untransfected HEK293T cells (green line) and transfected Human ENPP3 HEK293T stable cells (red line) were stained with anti-ENPP3 recombinant antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
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ENPP3 Recombinant Monoclonal Antibody

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Target Background

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