Product Details

Target Name

ectonucleotide pyrophosphatase/phosphodiesterase 3

Alternative Names

Alkaline phosphodiesterase I ELISA Kit; ARHR2 ELISA Kit; B10 ELISA Kit; CD203c ELISA Kit; CD203c antigen ELISA Kit; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ELISA Kit; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) ELISA Kit; E NPP 3 ELISA Kit; E-NPP 3 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase 3 ELISA Kit; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 ELISA Kit; ENPP3 ELISA Kit; ENPP3_HUMAN ELISA Kit; gp130RB13 6 ELISA Kit; gp130RB136 ELISA Kit; M6S1 ELISA Kit; NPP1 ELISA Kit; NPP3 ELISA Kit; NPPase ELISA Kit; NPPS ELISA Kit; Nucleotide pyrophosphatase ELISA Kit; PC 1 ELISA Kit; PC-1 ELISA Kit; PCA1 ELISA Kit; PD Ibeta ELISA Kit; PD-Ibeta ELISA Kit; PDNP1 ELISA Kit; PDNP3 ELISA Kit; Phosphodiesterase I beta ELISA Kit; Phosphodiesterase I/nucleotide pyrophosphatase 3 ELISA Kit; phosphodiesterase i/nucleotide pyrophosphatase beta ELISA Kit

Abbreviation

ENPP3

Uniprot No.

O14638

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ENPP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-101
1:2	Average %	99
	Range %	92-105
1:4	Average %	89
	Range %	83-94
1:8	Average %	91
	Range %	87-96

Recovery

The recovery of human ENPP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	98-109
EDTA plasma (n=4)	85	80-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.755	2.858	2.807	2.691
5	2.212	2.221	2.217	2.101
2.5	1.537	1.477	1.507	1.391
1.25	0.946	0.954	0.950	0.834
0.625	0.489	0.473	0.481	0.365
0.312	0.289	0.274	0.282	0.166
0.156	0.210	0.218	0.214	0.098
0	0.115	0.116	0.116

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human ENPP3 ELISA Kit was designed for the quantitative measurement of Human ENPP3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Background

Function
(From Uniprot)

Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Has also alkaline phosphodiesterase activity.

Gene References into Functions

We showed the ability of the immunological marker CD203c to predict the clinical efficacy of sublingual immunotherapy on rhinitis subjects allergic to Parietaria. PMID: 29702283
Basophil CD203c surface expression reliably discriminated cystic fibrosis with allergic bronchopulmonary aspergillosis from cystic fibrosis with Aspergillus colonization and cystic fibrosis over time. PMID: 26585435
Expression of CD203c on basophils as a marker of immunoglobulin E-mediated (L)-asparaginase allergy. PMID: 23581640
Anaphylactic transfusion reaction in homozygous haptoglobin deficiency detected by CD203c expression on basophils. PMID: 24497482
ENPP3 is a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb) PMID: 23960081
The early signaling requirements for the CD11b/CD203c compartment expression and CD63 degranulation provide support for the hypothesis that CD11b and CD203c reside in a similar compartment. PMID: 22722613
Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen PMID: 20975283
Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission PMID: 20159259
Influence of hyperosmotic conditions on basophil CD203c upregulation in patients with food-dependent exercise-induced anaphylaxis. PMID: 20047266
Data show that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils. PMID: 19418203
E-NPP3 is associated with carcinogenesis of human colon cancer and that serum E-NPP3 might be a tumor marker of colon carcinoma PMID: 14533006
E-NPP3 is involved in the infiltration of neoplastic bile duct carcinoma PMID: 15072822
Our data demonstrate that leptin promotes platelet activation, provides a mechanistic basis for the prothrombotic effect of this hormone, and identifies a potentially novel therapeutic avenue to limit obesity-associated cardiovascular disease. PMID: 15886225
In conclusion, using well-defined experimental conditions, the measurement of CD203c up-regulation on basophils in response to specific allergens is as reliable as CD63-BAT for the in vitro diagnosis of patients with IgE-mediated allergy. PMID: 17275019

Hide All

Subcellular Location

Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Secreted.

Protein Families

Nucleotide pyrophosphatase/phosphodiesterase family

Tissue Specificity

Detected on bile ducts in liver, and in blood serum (at protein level). Detected in prostate and uterus. Detected on basophils, but not neutrophils.

Database Links

HGNC: 3358

OMIM: 602182

KEGG: hsa:5169

STRING: 9606.ENSP00000350265

UniGene: Hs.486489

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-EL007681HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 3(ENPP3) ELISA kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background