GRSF1 Recombinant Monoclonal Antibody

GRSF1 (G-rich sequence factor 1) is an RNA-binding protein that plays essential roles in mitochondrial RNA metabolism and post-transcriptional gene regulation. This protein recognizes G-rich sequences in target RNAs and participates in mitochondrial ribosome biogenesis, making it a significant target for researchers investigating mitochondrial function, RNA processing, and cellular energy metabolism.

This recombinant monoclonal antibody against GRSF1 offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology using clone 8E7, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format, raised against a synthesized peptide from human GRSF1 protein, ensures reliable target recognition across your experimental timeline.

Validation studies demonstrate effective performance in flow cytometry applications using human samples. Testing in HepG2 cells, a widely used hepatocellular carcinoma line, showed clear positive signal separation from isotype control when cells were fixed, permeabilized, and stained at 1:100 dilution. This intracellular staining protocol confirms the antibody's utility for detecting GRSF1 in fixed cell populations, with recommended working dilutions ranging from 1:50 to 1:200 for flow cytometry applications. The antibody has also been validated for ELISA, providing flexibility for researchers requiring multiple detection platforms.

Supplied in a stabilized liquid format with glycerol for long-term storage stability, this antibody is well-suited for studies exploring mitochondrial biology, RNA-protein interactions, and cellular stress responses where GRSF1 function is implicated.