Code | CSB-RA695552A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The HGD recombinant monoclonal antibody is produced through a meticulous process involving several essential steps. Firstly, immunizing an animal with a synthesized peptide derived from human HGD and harvesting the B cells. Extracting total RNA from the harvested B cells and synthesizing cDNA. The cDNA is used as the template for the amplification of the HGD antibody genes through PCR. The HGD antibody genes are incorporated into an expression vector, which is transfected into host cells to enable antibody production. Subsequently, the HGD recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. This HGD recombinant monoclonal antibody can react with human protein in ELISA and IHC applications.
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