Code | CSB-RA011087A2HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
The production of the HSP90AA1 recombinant monoclonal antibody involves the application of DNA recombinant technology and in vitro genetic manipulation. Initially, an animal is immunized with a synthesized peptide derived from human HSP90AA1, leading to the isolation and selection of B cells that produce the desired antibody. These positive B cells undergo screening and single clone identification to ensure their specificity. Next, the light and heavy chains of the HSP90AA1 antibody are amplified through PCR and inserted into a plasmid vector, creating a recombinant vector. This vector is then introduced into a host cell line for efficient antibody expression. The HSP90AA1 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. With its ability to selectively bind to human HSP90AA1 protein, this antibody is well-suited for ELISA, WB, IHC, and IF applications.
The HSP90AA1 protein is a chaperone protein that plays an important role in cellular protein folding, maturation, and stability. It is involved in the folding and maintenance of a wide variety of client proteins, including kinases, transcription factors, and steroid hormone receptors, among others. In addition to its chaperone activity, HSP90AA1 also plays a role in signal transduction pathways by interacting with various signaling proteins and modulating their activity. It has been shown to play a critical role in the regulation of many key cellular processes, including cell cycle progression, cell survival, and apoptosis.
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