Product Details

Uniprot No.

Target Names

ILF3

Alternative Names

Interleukin enhancer-binding factor 3 (Double-stranded RNA-binding protein 76) (DRBP76) (M-phase phosphoprotein 4) (MPP4) (Nuclear factor associated with dsRNA) (NFAR) (Nuclear factor of activated T-cells 90 kDa) (NF-AT-90) (Translational control protein 80) (TCP80), ILF3, DRBF MPHOSPH4 NF90

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ILF3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7H8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ILF3, also known as NF90 or DRBP76, is a multifunctional double-stranded RNA-binding protein that plays essential roles in transcriptional regulation, mRNA stability, and translational control. As a key component of the nuclear factor of activated T-cells complex, ILF3 participates in immune response signaling and has emerged as a significant factor in cancer biology, where its dysregulation influences cell proliferation and tumor progression. The protein's involvement in both epigenetic regulation and signal transduction pathways makes it a compelling target for researchers investigating gene expression control mechanisms and disease pathogenesis.

This recombinant monoclonal antibody, clone 7H8, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human ILF3 and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. Affinity chromatography purification ensures high purity for reliable performance across applications.

Validation studies demonstrate robust performance in both immunohistochemistry and immunofluorescence applications. IHC testing on paraffin-embedded human ovarian cancer and cervical cancer tissues at 1:100 dilution using citrate buffer antigen retrieval confirms reliable detection in clinically relevant sample types. Immunofluorescence analysis of HeLa cells at 1:50 dilution reveals clear signal with appropriate subcellular localization patterns when counter-stained with DAPI.

Whether investigating ILF3's role in RNA metabolism, exploring its function in nuclear signaling cascades, or examining its expression patterns in cancer tissues, this antibody provides researchers with a dependable tool for advancing their understanding of this multifaceted regulatory protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs. As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response. Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs.

Gene References into Functions

DNA- and RNA-binding protein NF90/ILF3/NF110 associates with chromatin to hierarchically regulate transcription factors that promote proliferation and suppress differentiation. PMID: 29590119
Reprot a coordinated regulation of circRNA biogenesis and function by NF90/NF110 in viral infection. PMID: 28625552
The NF90 regulates PARP1 mRNA stability in hepatocellular carcinoma cells, and NF90 is a potential target to inhibit PARP1 activity. PMID: 28487110
Variants in ILF3 gene is associated with thrombosis. PMID: 26908601
these data establish miR-590-5p as an anti-onco-miR that inhibits colorectal cancer angiogenesis and metastasis through a new mechanism involving NF90/VEGFA signaling axis. PMID: 27735951
NF90 exerts its antiviral activity by antagonizing the inhibitory role of NS1 on PKR phosphorylation PMID: 27423063
These data suggest that, like ADAR2, underlying sequences in dsRNA may influence how NF90 recognizes its target RNAs PMID: 26712564
The NF90 C-terminus is involved in conformational changes in the protein after RNA binding. PMID: 26795062
Taken together, our discovery of the function of TCP80 and RHA in regulating p53 IRES and p53 induction following DNA damage provides a better understanding of the mechanisms that regulate IRES-mediated p53 translation in response to genotoxic stress. PMID: 26273641
data suggest that lncRNA SALNR modulates cellular senescence at least partly through changing NF90 activity PMID: 26491010
Data indicate the role of cyclin-dependent kinase inhibitor 1A (p21) and interleukin enhancer binding factor 3 (ILF3) pathway in mediating the anti-leukemic activity of sodium dichloroacetate (DCA) in oncoprotein p53null leukemic cells. PMID: 25544776
Suppression of NF90 caused a decrease in the half-life of cyclin E1 mRNA PMID: 25399696
the role of the human NF90 protein isoforms in DNA and RNA virus replication PMID: 25447144
NF90 exerts antiviral activity through regulation of PKR phosphorylation and stress granules in infected cells. PMID: 24623135
The function of NF90 in posttranscriptional gene regulation and microRNA biogenesis. [Review] PMID: 23965975
Data indicate that knockdown of interleukin enhancer-binding factor 3 (ILF3) leads to the increased levels of mature urokinase-type plasminogen activator (uPA) mRNA-targeting miRNAs miR-193a, miR-193b and miR-181a. PMID: 22986534
contributes to maintaining low levels of senescence-associated secretory phenotype factors in non-senescent cells PMID: 23117626
The unique C-terminal region of ILF3/NF110 is important for promoting survivin expression and for high affinity binding to YM155. PMID: 22442257
high expression of ERCC1, XPB and ILF3 was observed in human epithelial ovarian cancer PMID: 21971700
These data suggest that DRBP76, via its association with VP35, exerts an anti-Ebola virus function. PMID: 21987769
The protein polymorphism of Ilf3/NF90 and the various subcellular localizations of their isoforms may partially explain the various functions previously reported for these proteins. PMID: 21811582
associations of rs2569512 of ILF3 to myocardial infarction among individuals stratified by the absence or presence of hypertension, diabetes mellitus and chronic kidney disease PMID: 21347509
Production of HIV particles is regulated by altering sub-cellular localization and dynamics of Rev induced by double-strand RNA binding protein. PMID: 21364984
NF90 binds the dengue virus RNA 3' terminus and is a positive regulator of dengue virus. PMID: 21386893
The relationship between rs6929846 of BTN2A1 or rs2569512 of ILF3 and myocardial infarction is influenced by the serum concentrations of high density lipoprotein cholesterol and low density lipoprotein cholesterol, respectively. PMID: 21468600
Data suggest that physiologic DRBP76 expression, isoform distribution and subcellular localization are profoundly altered upon malignant transformation. PMID: 20668518
Nuclear export of this protein is required for interleukin-2 mRNA stabilization. PMID: 12504009
Results describe the expression of four isoforms of nuclear factor 90, including NF110, by cDNA cloning and mass spectrometric analysis of proteins isolated from human cells PMID: 12946349
nuclear export of ILF3 is mediated by exportin-5 and minihelix-containing RNA PMID: 14570900
the multiple cellular functions, i.e., translation control, interleukin-2 enhancer binding, or cell division, of ILF3 are fulfilled by alternatively spliced isoforms. PMID: 14654356
NF90 and NF110 engage RNA differentially and translocate from the nucleus to the cytoplasm in mitosis PMID: 15811368
ADAR1 has the potential both to change information content through editing of mRNA and to regulate gene expression through interacting with NF90 PMID: 16055709
The human Nuclear Factor 90 c-terminal variant protein encoded by ILF3 interacts with Rev to inhibit Rev-mediated RNA transport. PMID: 17125513
Our results show that perturbation Tat/TAR RNA interaction by the dsRNA binding nuclear factor 90 protein is sufficient to inhibit transcriptional activation of HIV-1. PMID: 17565699
DRBP76/NF90 isoform facilitates VEGF expression by promoting VEGF mRNA loading onto polysomes PMID: 18039850
NFARs exert influence on mRNA trafficking and the modulation of translation rates and may constitute an innate immune translational surveillance mechanism important in host defense countermeasures against virus infection PMID: 18337511
MKP-1 upregulation by oxidative stress is potently influenced by increased mRNA stability and translation, mediated at least in part by the RNA-binding proteins HuR and NF90. PMID: 18490444
ILF-3, which has been known to regulate IL-2 expression in T cells, up-regulates synoviolin expression with GABPalpha in rheumatoid synovial cells PMID: 19116932
NF90 inhibits influenza virus replication during the early phase of infection through direct interaction with viral NP. PMID: 19494010

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Subcellular Location

Nucleus, nucleolus. Cytoplasm. Nucleus.

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 6038

OMIM: 603182

KEGG: hsa:3609

STRING: 9606.ENSP00000404121

UniGene: Hs.465885

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Code	CSB-RA289890A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA289890A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA289890A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela Cells with CSB-RA289890A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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ILF3 Recombinant Monoclonal Antibody

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