Recombinant Human Novel Coronavirus Nucleoprotein(N)

In Stock
Code CSB-EP3325GMY
Size
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Western Blot
    Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
    SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
    Secondary
    Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific(CSB-PA160506) at 1/20000 dilution
    Predicted band size: 48 kDa
    Observed band size: 55 kDa
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A1GMY), the EC50 of SARS-CoV-2-N protein is 1.368 -1.804 ng/ml. Biological Activity Assay
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A0GMY), the EC50 of SARS-CoV-2-N protein is 4.267-5.568 ng/ml. Biological Activity Assay
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Product Details

Description
Recombinant Human Novel Coronavirus Nucleoprotein (N) (SARS-CoV-2) is a full length protein expressed with an N-terminal 6xHis-tagged in the E.coli. Its expression region corresponds to 1-419aa of Human Novel Coronavirus Nucleoprotein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band of 51.0 kDa on the gel. It was validated to be biologically active through WB and functional ELISA analyses. This recombinant SARS-CoV-2 N proteins in-stock are available.
N protein is encoded by the SARS-CoV-2 genome. It is highly immunogenic and abundantly expressed during infection. And it is often used in vaccine development and serological assay. After infection, the N protein together with the viral RNA is released into the host cell, promoting viral RNA package, viral particle assembly and release.
Purity Greater than 90% as determined by SDS-PAGE.
Activity ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A1GMY), the EC50 of SARS-CoV-2-N protein is 1.368 -1.804 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A0GMY), the EC50 of SARS-CoV-2-N protein is 4.267-5.568 ng/ml.
Target Names N (Nucleoprotein)
Uniprot No. P0DTC9
Research Area Microbiology
Alternative Names Nucleocapsid protein, NC, protein N, N
Species Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Source E.coli
Expression Region 1-419aa
Target Protein Sequence MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Mol. Weight 48.0kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Nucleoprotein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Coronavirus nucleoproteins are phosphoproteins, and are encoded near the 3′ end of the genome. N possesses two RNA-binding domains: an N-terminal domain with adjacent S/R-rich motif and the C-terminal 209 amino acids. N protein is invovled in coronavirus infection with many ways: the C-terminal domain (CTD) of N is important for binding the genomic RNA packaging signal leading to selective genome incorporation, the N3 domain interacts with the endodomain of M to form virions, and the serine–arginine repeat region of N (SR) interacts with the first ubiquitin-like domain of nsp3 in a critical early replication step. Moreover, it has also been demonstrated that N can oligomerize through interactions in the CTD, bind viral RNA through the N-terminal domain, unwind double-stranded nucleic acid in the manner of an RNA chaperone, and pack in a helix through the N-terminal domain, though none of these other functions has yet been demonstrated to be important for infection.
Subcellular Location Virion i UniRule annotation, Host endoplasmic reticulum-Golgi intermediate compartment i UniRule annotation, Host Golgi apparatus
Protein Families Betacoronavirus nucleocapsid protein family

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