Restriction Site Finder Tool

Enter a nucleic acid sequence to quickly find and count restriction sites. Results will show enzyme name, recognition sequence, cut site, and occurrence count.

Enter Nucleic Acid Sequence

Please enter an ATCG sequence in uppercase letters. N is supported as any base.

Instructions

Enter Sequence

Enter your DNA sequence in the input box using uppercase ATCG. N can be used as any base.

Click Find Button

Click the "Find Restriction Sites" button to analyze the sequence.

View Results

Results will be shown in the table, including enzyme name, recognition sequence, cut site, and count.

Notes

  • Sequence length is recommended to be less than 10,000 bases for best performance
  • Arrows in the cut site indicate cleavage position, "⇅" means palindromic cut
  • "N" in the sequence means any base (A, T, C, G)
  • Position numbers start from 1
Video Tutorial

Check out the video tutorial below for a quick walkthrough.

Enzyme List

Below is the list of supported restriction sites. Total:

Name Recognition Sequence Cut Site
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