- DNA/Protein Repeat Sequence Finder — Identify tandem repeats in your insert sequence before cloning; repeat regions may affect restriction enzyme accessibility
- Protein Physicochemical Parameter Calculator — Predict key properties of the protein your cloned gene will produce
Enter Nucleic Acid Sequence
Please enter an ATCG sequence in uppercase letters. N is supported as any base.
Instructions
Enter Sequence
Enter your DNA sequence in the input box using uppercase ATCG. N can be used as any base.
Click Find Button
Click the "Find Restriction Sites" button to analyze the sequence.
View Results
Results will be shown in the table, including enzyme name, recognition sequence, cut site, and count.
Notes
- Sequence length is recommended to be less than 10,000 bases for best performance
- Arrows in the cut site indicate cleavage position, "⇅" means palindromic cut
- "N" in the sequence means any base (A, T, C, G)
- Position numbers start from 1
Check out the video tutorial below for a quick walkthrough.
Enzyme List
Below is the list of supported restriction sites. Total:
| Name | Recognition Sequence | Cut Site |
|---|
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Citing This Tool
If you use the CUSABIO Restriction Site Finder in your research, please cite it as follows:
CUSABIO Technology LLC. Restriction Site Finder Tool [Online Tool]. Available at: https://www.cusabio.com/Restriction-Site-Finder-Tool.html (accessed [Month Year]).
For methods sections, you may describe the tool as:
"Restriction endonuclease site mapping was performed using the CUSABIO Restriction Site Finder (https://www.cusabio.com/Restriction-Site-Finder-Tool.html), a browser-based tool that identifies restriction sites across the input DNA sequence and reports enzyme name, recognition sequence, cut site position, and occurrence count."
