Recombinant Escherichia coli Lipopolysaccharide biosynthesis protein wzzE(wzzE)

Code CSB-CF365386ENV
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names wzzE
Uniprot No. P0AG00
Alternative Names
wzzE; wzz; yifC; b3785; JW5601; ECA polysaccharide chain length modulation protein
Species Escherichia coli (strain K12)
Expression Region 1-348
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Modulates the polysaccharide chain length of enterobacterial common antigen (ECA). Required for the assembly of the phosphoglyceride-linked form of ECA (ECA(PG)) and the water-soluble cyclic form of ECA (ECA(CYC)).
Gene References into Functions
  1. WzzE is not involved in the synthesis of lipopolysaccharide but participates in the pathogenic process. PMID: 29727150
  2. E.coli WzzB and WzzE Polysaccharide Copolymerases form octameric homo-oligomeric complexes. PMID: 25307743
  3. Results suggest that the regions predicted to be coiled coils are important for Wzz function because they maintain the native conformation of the protein, although the existence of coiled coils could not be demonstrated experimentally. PMID: 18203821
Subcellular Location Cell inner membrane; Multi-pass membrane protein.
Protein Families WzzB/Cld/Rol family
Database Links

KEGG: ecj:JW5601

STRING: 316385.ECDH10B_3974

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