Recombinant Human Potassium voltage-gated channel subfamily KQT member 4(KCNQ4)

Code CSB-CF012092HU
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names KCNQ4
Uniprot No. P56696
Alternative Names KCNQ4; Potassium voltage-gated channel subfamily KQT member 4; KQT-like 4; Potassium channel subunit alpha KvLQT4; Voltage-gated potassium channel subunit Kv7.4
Species Homo sapiens (Human)
Expression Region 1-695
Target Protein Sequence MAEAPPRRLGLGPPPGDAPRAELVALTAVQSEQGEAGGGGSPRRLGLLGSPLPPGAPLPG PGSGSGSACGQRSSAAHKRYRRLQNWVYNVLERPRGWAFVYHVFIFLLVFSCLVLSVLST IQEHQELANECLLILEFVMIVVFGLEYIVRVWSAGCCCRYRGWQGRFRFARKPFCVIDFI VFVASVAVIAAGTQGNIFATSALRSMRFLQILRMVRMDRRGGTWKLLGSVVYAHSKELIT AWYIGFLVLIFASFLVYLAEKDANSDFSSYADSLWWGTITLTTIGYGDKTPHTWLGRVLA AGFALLGISFFALPAGILGSGFALKVQEQHRQKHFEKRRMPAANLIQAAWRLYSTDMSRA YLTATWYYYDSILPSFRELALLFEHVQRARNGGLRPLEVRRAPVPDGAPSRYPPVATCHR PGSTSFCPGESSRMGIKDRIRMGSSQRRTGPSKQHLAPPTMPTSPSSEQVGEATSPTKVQ KSWSFNDRTRFRASLRLKPRTSAEDAPSEEVAEEKSYQCELTVDDIMPAVKTVIRSIRIL KFLVAKRKFKETLRPYDVKDVIEQYSAGHLDMLGRIKSLQTRVDQIVGRGPGDRKAREKG DKGPSDAEVVDEISMMGRVVKVEKQVQSIEHKLDLLLGFYSRCLRSGTSASLGAVQVPLF DPDITSDYHSPVDHEDISVSAQTLSISRSVSTNMD
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinic receptors.
Gene References into Functions
  1. KCNQ4 gene polymorphisms associated with susceptibility to noise-induced hearing loss. PMID: 29072670
  2. mutations in KCNQ4 gene are unlikely to be a major causative factor of ADNSHL in our studied patients from West Bengal, India, pointing to other genes might be responsible for ADNSHL in our studied patients PMID: 28802383
  3. The fundamental processes that dictate Kv7.4 activity. PMID: 27981364
  4. A novel KCNQ4 mutation, c.887 G > A (p.G296D), was identified in all five affected members in a Chinese family with autosomal dominant non-syndromic deafness 2. This mutation leads to a glycine-to-aspartic acid substitution at position 296 in the pore region of the KCNQ4 channel. PMID: 28340560
  5. Tannic acid activates Kv7.4 and Kv7.3/7.5 K(+) channels resulting in vasodilation. PMID: 26969140
  6. Gene and protein expression analyses show the predominance of KV7.4 channels over the other KV7 channel subtypes in human detrusor. PMID: 27761601
  7. Kv7.4 channels are present and functional in cardiac mitochondria; their activation exerts a significant cardioprotective role PMID: 26718475
  8. analysis of mechanistic insights into the critical roles of Ca(2+)/CaM regulation of the Kv7.4 channel under physiological and pathological conditions PMID: 26515070
  9. Interaction between G-protein betagamma subunits and Kv7.4 is crucial for channel responses to membrane voltage. PMID: 25941381
  10. genotype-phenotype correlation is analogous to that in KCNQ1 which causes autosomal dominant hereditary long QT syndrome 1 with milder phenotype and the autosomal recessive Jervell and Lange-Nielsen syndrome 1 with more severe phenotype PMID: 26036578
  11. This study provides evidence that mouse Kv7 channels may contribute differently to regulating the functional properties of cerebral and coronary arteries. PMID: 25476662
  12. The study identified a novel KCNQ4 mutation in a five generation Chinese family and a known KCNQ4 mutation in a six generation Chinese family. PMID: 25116015
  13. These findings suggest a protective role for Kv7.4 channels in the pulmonary circulation, limiting its reactivity to pressor agents and preventing hypoxia-induced pulmonary hypertension. PMID: 25361569
  14. Identified the c.211delC mutation in the KCNQ4 gene and the c.2967C>A (p.H989Q) mutation in the TECTA gene to be associated with high-frequency sensorineural hearing loss in a Japanese family. PMID: 24655070
  15. Kv7.4 currents are inhibited in a CB1 pathway repressed by endocannabinoid 2-AG PMID: 24927567
  16. In-frame deletion in KCNQ4 P-loop was identified in family members with autosomal dominant sensorineural hearing loss. PMID: 23443030
  17. Differential protein kinase C-dependent modulation of Kv7.4 and Kv7.5 subunits of vascular Kv7 channels. PMID: 24297175
  18. decreased cell surface expression and impaired conductance of the KCNQ4 channel are two mechanisms underlying hearing loss in DFNA2 PMID: 23750663
  19. A new typical audiogram configuration characterized by mid-frequency predominant hearing loss caused by the KCNQ4 V230E mutation. PMID: 23717403
  20. The present study supports the idea that a non-truncating mutation around the N-terminus of KCNQ4 pore helix may be related to moderate hearing loss. PMID: 23399560
  21. KCNQ4 surface expression was restored by HSP90beta in cells mimicking heterozygous conditions of the DFNA2 patients. PMID: 23431407
  22. The data of this study identified a dynamic redox sensor within neuronal M-channels, which mediates reciprocal regulation of channel activity by NO and ROS PMID: 23554485
  23. Data indicate a missense mutation encoding a Tyr270His located at the N-terminus of the highly conserved of KCNQ4 pore helix sequence. PMID: 22420747
  24. no sequence alterations that segregate with autosomal dominant non-syndromic deafness in either GJB3 or KCNQ4 PMID: 21651318
  25. This work describes a gene mutation that modulates touch sensitivity in mice and humans and establishes KCNQ4 as a specific molecular marker for rapidly adapting Meissner and a subset of hair follicle afferents. PMID: 22101641
  26. Data show that KCNQ4 and KCNE1 isoforms were suppressed in placentas from term preeclamptic women. PMID: 21730298
  27. Identified is novel mutation (c.664_681del) in KCNQ4 associated with hearing loss in a Korean family with dominantly inherited deafness. PMID: 20832469
  28. mutations in the pore region, namely L274H, W276S, L281S, G285C, and G296S, as well as the C-terminal mutant G321S in the heterologous expression system, yielded non-functional channels because of endoplasmic reticulum retention of the mutant channels PMID: 20966080
  29. Autosomal dominant progressive sensorineural hearing loss due to a novel mutation in the KCNQ4 gene. PMID: 21242547
  30. evidence of the cellular etiology and mechanisms of SGN degeneration in DFNA2. PMID: 20739290
  31. The W276S mutation has occurred three times independently, and most likely represents a hot spot for mutation in the KCNQ4 gene. PMID: 12112653
  32. DFNA2 locus was found to be related to hereditary sensorineural hearing loss. PMID: 12484650
  33. KCNQ3 interacts with KCNQ4. . A chimaera (KCNQ1-sid(Q3)) carrying the si domain of KCNQ3 within the KCNQ1 backbone interacted with KCNQ4. PMID: 12524525
  34. Src associates with KCNQ2-5 subunits but phosphorylates only KCNQ3-5. PMID: 15304482
  35. KCNQ4 phosphorylation via PKA and coupling to a complex that may include prestin can lead to the negative activation and the negative resting potential found in adult outer hair cells. PMID: 15660259
  36. Polymorphisms within the KCNQ4 gene are associated with susceptibility Noise-induced hearing loss. PMID: 16823764
  37. Results shows KCNQ4 SNPs were significantly associated with Age-related hearing impairment. PMID: 16917933
  38. a novel mutation of KCNQ4 gene was identified in patients with nonsyndromic deafness. PMID: 17033161
  39. In conclusion, this work demonstrates that inactivation is a key regulatory mechanism of Kv7.4 and Kv7.5 channels. PMID: 17237198
  40. high-resolution structure of the Kv7.4 A-domain Tail together with biochemical experiments show that the domain is a self-assembling, parallel, four-stranded coiled coil PMID: 17329207
  41. G296S mutant exerts a strong dominant-negative effect on potassium currents by reducing the wild type KCNQ4 channel expression at the cell surface PMID: 18030493
  42. among the allowed assembly conformations are KCNQ3/4 and KCNQ4/5 heteromers. PMID: 18786918
  43. KCNQ4 mutations associated with progressive sensorineural hearing loss. PMID: 18797286
  44. Two novel missense mutations and a stop mutation were detected in three American families predicted to have DFNA2-related deafness; The latter is the first DFNA2-causing stop mutation reported in KCNQ4. PMID: 18941426

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Involvement in disease Deafness, autosomal dominant, 2A (DFNA2A)
Subcellular Location Basal cell membrane; Multi-pass membrane protein.
Protein Families Potassium channel family, KQT (TC 1.A.1.15) subfamily, Kv7.4/KCNQ4 sub-subfamily
Tissue Specificity Expressed in the outer, but not the inner, sensory hair cells of the cochlea. Slightly expressed in heart, brain and skeletal muscle.
Database Links

HGNC: 6298

OMIM: 600101

KEGG: hsa:9132

STRING: 9606.ENSP00000262916

UniGene: Hs.473058

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