Recombinant Mouse Apolipoprotein C-I (Apoc1)

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Code CSB-CF001930MO
Size $878
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Apoc1
Uniprot No.
Research Area
Cardiovascular
Alternative Names
Apoc1; Apolipoprotein C-I; Apo-CI; ApoC-I; Apolipoprotein C1
Species
Mus musculus (Mouse)
Source
in vitro E.coli expression system
Expression Region
27-88aa
Target Protein Sequence
APDLSGTLESIPDKLKEFGNTLEDKARAAIEHIKQKEILTKTRAWFSEAFGKVKEKLKTTFS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
12.5 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

I want to confirm the concentration is 0.4mg/ml. Can we dilute using DMEM f12 to our desired concentrations? Are there any best handling practices we should be aware of?

A:

The lyophilized can be reconstituted with deionized sterile water. If a large amount of dilution is needed, it needs to be diluted with a corresponding buffer. For example, CSB-BP018193HU is provided with Tris-buffer, then please use Tris-buffer for dilution.

Target Background

Function
Inhibitor of lipoprotein binding to the low density lipoprotein (LDL) receptor, LDL receptor-related protein, and very low density lipoprotein (VLDL) receptor. Associates with high density lipoproteins (HDL) and the triacylglycerol-rich lipoproteins in the plasma and makes up about 10% of the protein of the VLDL and 2% of that of HDL. Appears to interfere directly with fatty acid uptake and is also the major plasma inhibitor of cholesteryl ester transfer protein (CETP). Modulates the interaction of APOE with beta-migrating VLDL and inhibits binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification.
Gene References into Functions
  1. APOC1 expression induces glomerulosclerosis, potentially by increasing the cytokine response in macrophages. PMID: 27976371
  2. Apolipoprotein C-I was significantly increased in obese mice plasma. PMID: 22404376
  3. The absence of ApoC-I results in impaired memory functions, which is, together with previous data, suggestive of an important, bell-shaped gene-dose dependent role for ApoC-I in appropriate brain functioning PMID: 21157034
  4. Data show that the stimulating effect of apoCI on the lipopolysaccharide (LPS)response resembles that of LPS-binding protein (LBP) and depends on CD14/ Toll-like receptor 4 signaling. PMID: 20335569
  5. regulated expression of gene cluster in macrophages PMID: 12032151
  6. present observations provide direct support for a potent specific inhibition of CETP by plasma apoCI in vivo PMID: 12070157
  7. The apoC-I content of lipoprotein remnants may serve as an early marker of coronary artery disease risk. PMID: 12231568
  8. TR4 can also regulate apolipoprotein E, C-I, and C-II gene expression via the TR4 response element within the hepatic control region PMID: 12954636
  9. apoC-I is a potent inhibitor of LPL-mediated triglyceride lipolysis PMID: 15576844
  10. irrespective of receptor-mediated remnant clearance by the liver, liver-specific expression of recombinant human apoCI causes hypertriglyceridemia in the absence of the VLDLr and apoCIII in mice PMID: 16478678
  11. Endogenous apoC-I increases hyperlipidemia in apoE-knockout mice by stimulating VLDL production and inhibiting LPL. PMID: 16537968
  12. Knockout mice show significant increases in the hepatic content of cholesteryl esters and triglycerides and in biliary cholesterol concentration. PMID: 17053273
  13. Systemic apoCI increases atherosclerosis, probably by inducing hyperlipidemia, in the absence of ApoE PMID: 17320883
  14. Data suggest that apoC-I binds free fatty acids (FFA), thereby reducing the blood FFA for uptake by cells, and this mechanism can serve as an additional mechanism for the resistance to obesity and the cutaneous abnormalities of APOC1(+/+) mice. PMID: 17339654
  15. apoCI is crucially involved in lipopolysaccharide(LPS)-induced atherosclerosis in apoe-/- mice, which mainly relates to an increased inflammatory response toward LPS PMID: 17967778
  16. apoCI is a novel inhibitor of the scavenger receptor BI -mediated uptake of HDL cholesterol by hepatocytes; apoCI is a determinant for the plasma levels and size of HDL in vivo. PMID: 18992221

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Subcellular Location
Secreted.
Protein Families
Apolipoprotein C1 family
Tissue Specificity
Adult and fetal liver.
Database Links
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