Recombinant Mouse Tyrosine-protein phosphatase non-receptor type 5(Ptpn5)

Code CSB-CF019042MO
Size Pls inquire other sizes
Source in vitro E.coli expression system
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Product Details

Target Names Ptpn5
Uniprot No. P54830
Alternative Names Ptpn5; Tyrosine-protein phosphatase non-receptor type 5; EC 3.1.3.48; Neural-specific protein-tyrosine phosphatase; Striatum-enriched protein-tyrosine phosphatase; STEP
Species Mus musculus (Mouse)
Expression Region 1-541
Target Protein Sequence MCCSERLLGLPQPVEMEAPDEAEGLPSKQKEMPPPPPPSPPSEPAQKLPPQGAGSHSLTVRSSLCLFAASQFLLACGVLWLSGHGHSWLQNTTDLISSSLTVLNHLGPVAWLGSGTWGIPSLLLVSLTVSLVIVTTLVWHLLKAPPEPPAPLPPEDRRQSVSRQPSFTYSEWMEEKVEDDFLDLDAVPETPVFDCVMDIKPETDPASLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLVSPREESAHEYLLSASRVLRAEELHEKALDPFLLQAEFFEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVRLTSPDPEDPLSSYINANYIRGYSGEEKVYIATQGPIVSTVADFWRMVWQERTPIIVMITNIEEMNEKCTEYWPEEQVVHDGVEITVQKVIHTEDYRLRLISLRRGTEERSLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCSPIIVHCSAGIGRTGCFIATSICCQQLRREGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHAMSLYEKQLSLQSSE
Protein Length full length protein
Tag Info The following tags are available.
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
Gene References into Functions
  1. STEP plays an important role at nerve terminals in the regulation of Ca(2+) homeostasis and neurotransmitter release. PMID: 29508281
  2. STEP61 degradation is an important event in BDNF-mediated effects. PMID: 26223799
  3. STEP has a key role in the regulation of psychomotor action and physical dependency to morphine. These data suggest that STEP inhibition may be a critical target for the treatment of withdrawal symptoms associated with morphine. PMID: 26915673
  4. This study demonistrated that behavioral, molecular, and electrophysiological data indicate that spinal STEP61 plays a regulatory role in nociception. PMID: 26270590
  5. STEP61 regulates BDNF expression, with implications for cognitive functioning in CNS disorders. PMID: 26450419
  6. STEP contributes, at least in part, to the protection against the ingestion of aversive agents. PMID: 25992601
  7. These results indicate that STEP61 is a novel substrate of parkin, although further studies are necessary to determine whether elevated STEP61 levels directly contribute to the pathophysiology of PD. PMID: 25583483
  8. Abeta regulating STEP61 activity is mediated by Abeta binding to alpha7 nAChRs PMID: 24123152
  9. Increased STEP and calpain activation contribute to altered NMDAR localization in an Huntington's disease mouse model. PMID: 22523092
  10. genetically reducing STEP significantly diminishes seizures and restores select social and nonsocial anxiety-related behaviors in Fmr1(KO) mice PMID: 22405502
  11. Increased STEP61 plays a role in amyloid Abeta-mediated internalization of the (AMPAR) subunits GluA1/GluA2 (formerly GluR1/GluR2). PMID: 21883219
  12. The absence of STEP improves cognitive performance, and may do so by the regulation of downstream effectors necessary for synaptic transmission. PMID: 21501258
  13. STEP pathway is severely downregulated in the presence of mutant huntingtin and may participate in compensatory mechanisms activated by striatal neurons that lead to resistance to excitotoxicity. PMID: 21632937
  14. STEP may be required for ethanol's amnesic effects. PMID: 21464302
  15. Electrical stimulation of the hippocampal-entorhinal cortex pathway in STEP knockout mice resulted in less activation of the dentate gyrus granule cell layer (GCL), but greater activation of the hilus in STEP knockouts, compared with heterozygous slices. PMID: 21204826
  16. genetic manipulations to reduce STEP activity in a triple transgenic AD mouse model and show that a decrease in STEP levels reverses cognitive and cellular deficits observed in these mice. PMID: 20956308
  17. These results suggest that STEP is an important mediator in the dopamine D2R-mediated activation of ERK signaling and in the regulation of dopaminergic neuronal development. PMID: 18708052
  18. results reveal a novel mechanism by which Abeta-mediated accumulation of STEP61 results in increased internalization of NR1/NR2B receptor that may contribute to the cognitive deficits in Alzheimer's disease PMID: 20427654
  19. STEP increases the sensitivity of neurons to status epilepticus-induced excitotoxicity by specifically blocking a latent neuroprotective response initiated by the mitogen-activated protein kinase pathway. PMID: 17360923
  20. STEP regulates alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization in hippocampal slices, synaptoneurosomes and primary neuronal cultures. PMID: 18923032
  21. Striatal-enriched protein tyrosine phosphatase (STEP) knockout mice show enhanced phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) in the striatum, hippocampus and amygdala. PMID: 18932218
  22. These findings suggest a novel mechanism by which differential NMDAR stimulation regulates STEP(61) to promote either ERK1/2 or p38 activation and identifies calpain cleavage of STEP(61) as a valid target for the development of neuroprotective therapy. PMID: 19625523

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Subcellular Location Isoform STEP61: Endoplasmic reticulum membrane, Multi-pass membrane protein, SUBCELLULAR LOCATION: Isoform STEP46: Cytoplasm
Protein Families Protein-tyrosine phosphatase family, Non-receptor class subfamily
Tissue Specificity STEP20 is expressed only in the CNS.
Database Links

KEGG: mmu:19259

STRING: 10090.ENSMUSP00000033142

UniGene: Mm.4654

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