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Tumor Necrosis Factor
Recombinant Human Tumor necrosis factor receptor superfamily member 10C (TNFRSF10C), partial (Active)

Recombinant Human Tumor necrosis factor receptor superfamily member 10C (TNFRSF10C), partial (Active)

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Code	CSB-AP004961HU
Abbreviation	Recombinant Human TNFRSF10C protein, partial (Active)
MSDS	MSDS Datasheet
Size	$78
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

The ED50 as determined by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL is 0.56 ng/ml.

Target Names

TNFRSF10C

Uniprot No.

O14798

Research Area

Cancer

Alternative Names

TNFRSF10C; DCR1; LIT; TRAILR3; TRID; UNQ321/PRO366; Tumor necrosis factor receptor superfamily member 10C; Antagonist decoy receptor for TRAIL/Apo-2L; Decoy TRAIL receptor without death domain; Decoy receptor 1; DcR1; Lymphocyte inhibitor of TRAIL; TNF-related apoptosis-inducing ligand receptor 3; TRAIL receptor 3; TRAIL-R3; TRAIL receptor without an intracellular domain; CD antigen CD263

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

26-221aa

Complete Sequence

ATTARQEEVPQQTVAPQQQRHSFKGEECPAGSHRSEHTGACNPCTEGVDYTNASNNEPSCFPCTVCKSDQKHKSSCTMTRDTVCQCKEGTFRNENSPEMCRKCSRCPSGEVQVSNCTSWDDIQCVEEFGANATVETPAAEETMNTSPGTPAPAAEETMNTSPGTPAPAAEETMTTSPGTPAPAAEETMTTSPGTPA

Mol. Weight

21.78 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.2

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Datasheet & COA

Please contact us to get it.

Description

TNFRSF10C functions as a decoy receptor that competitively sequesters TRAIL without transducing apoptotic signals, making it a critical tool for dissecting TRAIL pathway regulation in cancer biology. This recombinant construct spans the extracellular ligand-binding domain (aa 26–221), expressed in mammalian cells to preserve native disulfide bonding and glycosylation essential for proper TRAIL engagement. Functional validation demonstrates an ED50 of 0.56 ng/mL in inhibiting TRAIL-mediated cytotoxicity in L-929 fibroblasts, confirming robust competitive inhibition activity that supports use in ligand-binding interaction assays (SPR, BLI, ELISA), TRAIL-blocking antibody screening, and therapeutic antibody epitope mapping. The preparation satisfies purity and endotoxin criteria typical for cell-based assays, with greater than 95% purity by SDS-PAGE and endotoxin levels below 1.0 EU/μg, providing a suitable basis for drug discovery screens targeting TRAIL-decoy receptor interactions.

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Target Background

Function

Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand.

Gene References into Functions

High levels of TRAIL-R3 and CCR-2 expression in tumor epithelial cells identified patients with early breast cancer with poor outcomes. PMID: 28420351
DcR1 levels in serum sample which were significantly lower in AMD patients. PMID: 24534820
DcR1 upregulation mediates temozolomide resistance. PMID: 25808868
GATA4 and DcR1 promoter hypermethylation is tumor specific event in glioblastoma but they promoter methylation cannot be considered as a prognostic marker of glioblastoma survival. PMID: 23320456
Primary EOC is associated to a lower TRAIL-R3 expression. PMID: 22555108
Data show that about 20% of AML patients highly expressed decoy receptor TRAIL-R3, which was strongly correlated to a shortened overall survival. PMID: 21281967
Decoy receptors DcR1 and DcR2 on CD8+ T cells, but not on CD4-positive T cells, are positively correlated with patients' DAS scores. PMID: 20799941
Results suggest that DcR1 expression occurs in a subset of endometrial carcinomas and may contribute to resistance to TRAIL-induced apoptosis. PMID: 19936781
analysis of the transcription initiation sites and promoter structure of the TRAIL-R3 gene PMID: 12417331
cloned and characterized a p53 consensus element located within the first intron of the TRAIL-R3 gene PMID: 14623878
Our results demonstrate that DcR1 and DcR2 genes are frequently methylated in various tumor types, and aberrant methylation was the cause for silencing of DcR1 and DcR2 expression. PMID: 14999791
The DcR1 had no death domain and was anchored to the membrane via a glycophosphatidyl inositol tail. PMID: 15538968
Resistance to TRAIL-induced apoptosis in acute myeloid leukemia cells is associated with expression of TRAIL-R3. PMID: 15921376
tryptophol induces apoptosis through DR5 and the resistance of PBL to tryptophol-induced apoptosis might be due to competition from DcR1 PMID: 17690453
p53 negatively regulates oxaliplatin-mediated TRAIL-induced apoptotic activity through DcR1 upregulation. PMID: 18345033
TRAILR3 (TNF-related apoptosis inducing ligand receptor 3) levels were significantly higher in lung parenchyma in subjects with emphysema PMID: 18511705
Liver metastases with low DcR1/TNFRSF10C mRNA expression were more likely to present with extrahepatic metastases PMID: 18590575
inactivation of TNFRSF10C by chromosomal deletion and promoter methylation may play an important role in prostate cancer development PMID: 19035483
Low DCR1 is associated with non-small cell lung cancer. PMID: 19661294

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity

Higher expression in normal tissues than in tumor cell lines. Highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart.

Database Links

HGNC: 11906

OMIM: 603613

KEGG: hsa:8794

STRING: 9606.ENSP00000349324

UniGene: Hs.655801

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