Code | CSB-E09315h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human TACE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 84 | ||||
Range % | 80-90 | |||||
1:2 | Average % | 97 | ||||
Range % | 92-103 | |||||
1:4 | Average % | 92 | ||||
Range % | 86-99 | |||||
1:8 | Average % | 93 | ||||
Range % | 85-98 |
The recovery of human TACE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-100 | ||||
EDTA plasma (n=4) | 97 | 90-103 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Based on the Sandwich-ELISA principle and enzyme-substrate chromogenic reaction property, the users can measure the content of mouse ADAM17 in serum, plasma, tissue homogenates, and cell culture supernates. This mouse ADAM17 ELISA kit is quality tested with high specificity, excellent sensitivity, the precision of less than 10%, good linearity, and high recovery.
ADAM17, also called TACE, is a type I transmembrane metalloproteinase that is involved in the shedding of the extracellular domain of several transmembrane proteins such as cytokines, growth factors, receptors, and adhesion molecules thus regulating these crucial signaling pathways. It participates in various physiological and pathophysiological processes, including development, regeneration, differentiation, immunity, inflammation, neurodegeneration, fibrosis, and cancer progression. Overexpression of ADAM17 has been linked to invasion and metastasis in a variety of malignancies. Studies have shown that ADAM17 plays a potential role in breast cancer, including cell proliferation, invasion, angiogenesis, apoptosis, and therapy resistance.
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