AFF4 Antibody

1. The TAR central loop contacts the CycT1 Tat-TAR recognition motif (TRM) and the second Tat Zn(2+)-binding loop. Hydrogen-deuterium exchange (HDX) shows that AFF4 helix 2 is stabilized in the TAR complex despite not touching the RNA, explaining how it enhances TAR binding to the SEC 50-fold. PMID: 27731797
2. AFF4 is positioned to make unexpected direct contacts with HIV Tat, and Tat enhances P-TEFb/CCNT1 affinity for AFF4. PMID: 23471103
3. missense mutations in AFF4 in three unrelated probands with a new syndrome that phenotypically overlaps Cornelia de Lange syndrome (CdLS) that was named CHOPS syndrome (C for cognitive impairment and coarse facies, H for heart defects, O for obesity, P for pulmonary involvement and S for short stature and skeletal dysplasia) PMID: 25730767
4. HIV-1 Tat recruits transcription elongation factors dispersed along a flexible AFF4 scaffold. PMID: 23251033
5. overexpression of AFF2/3/4 interferes with the organization and/or biogenesis of nuclear speckles. PMID: 21330300
6. Through the bridging functions of Tat and AFF4, P-TEFb and ELL2 combine to form a bifunctional elongation complex that greatly activates HIV-1 transcription. PMID: 20471948
7. AFF4 is required for super elongation complex stability and proper transcription by poised RNA polymerase II. PMID: 20159561
8. MCEF has 21 exons & 7 nuclear localization sequence. 3 protein sequences, coded by 3 exons, mediate nuclear localization. Ectopic expression of MCEF repressed HIV-1 LTR-directed RNA polymerase II transcription, at the level of Tat-transactivation. PMID: 17389929
9. Data describe the cloning and expression of MCEF, a member of the AF4 family of transcription factors involved in acute lymphoblastic leukemia. [MCEF] PMID: 12065898

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HGNC: 17869

OMIM: 604417

KEGG: hsa:27125

STRING: 9606.ENSP00000265343

UniGene: Hs.519313