TAF9 Antibody

Datasheet

Code	CSB-PA619078ESR1HU
Size	US$166
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Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, 293T whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, A431 whole cell lysate All lanes: AK6 antibody at 0.59μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21, 20 kDa Observed band size: 21 kDa Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA619078ESR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA619078ESR1HU at dilution of 1:100
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TAF9 Polyclonal antibody

Uniprot No.

Q16594

Target Names

TAF9

Alternative Names

TAF9 antibody; TAF2G antibody; TAFII31 antibody; Transcription initiation factor TFIID subunit 9 antibody; RNA polymerase II TBP-associated factor subunit G antibody; STAF31/32 antibody; Transcription initiation factor TFIID 31 kDa subunit antibody; TAFII-31 antibody; TAFII31 antibody; Transcription initiation factor TFIID 32 kDa subunit antibody; TAFII-32 antibody; TAFII32 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Transcription initiation factor TFIID subunit 9 protein (1-264AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.

Gene References into Functions

TAF9 directly binds HCA587/MAGEC2 through its CR domain, suggesting that HCA587/MAGEC2 may involve in TAF9 binding with DNA to regulate certain genes transcription. PMID: 29257297
findings suggest that p53 sequesters TAF9 from GLI1, which may contribute to inhibition of GLI1 activity by p53 PMID: 26282181
TAF9 depletion severely disrupts TFIID, indicating that the observed ongoing transcription is initiated with at least partially TAF-free TATA-binding protein. PMID: 12837753
IL-1-inducible phosphorylation of p65 NFkB is mediated by multiple protein kinases including IKKalpha, IKKbeta, IKKepsilon, TBK1, and an unknown kinase and couples p65 to TAFII31-mediated IL-8 transcription PMID: 15489227
suggest a novel STAF65gamma-dependent function of STAGA-type complexes in cell proliferation and transcription activation by MYC postloading of TFIID and RNA polymerase II that involves direct recruitment of core Mediator PMID: 17967894
TAF9 functionally supports EKLF activity by enhancing its ability to activate the beta-globin gene. TAF9 interacts with a conserved beta-globin downstream promoter element PMID: 19251649
Multiple hTAF(II)31-binding motifs in the intrinsically unfolded transcriptional activation domain of VP16. PMID: 19643037

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Subcellular Location

Nucleus.

Protein Families

TAF9 family

Database Links

HGNC: 11542

OMIM: 600822

KEGG: hsa:6880

STRING: 9606.ENSP00000217893

UniGene: Hs.653163

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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