AKAP7 Antibody

Datasheet

Code	CSB-PA001529LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA001529LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA001529LA01HU at dilution of 1:100 Immunofluorescence staining of HepG2 cells with CSB-PA001529LA01HU at 1:120,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) AKAP7 Polyclonal antibody

Uniprot No.

O43687

Target Names

AKAP7

Alternative Names

A kinase (PRKA) anchor protein 7 antibody; A kinase anchor protein 18 kD antibody; A kinase anchor protein 7 antibody; A kinase anchor protein 7 isoform alpha antibody; A kinase anchor protein 7 isoform beta antibody; A kinase anchor protein 7 isoform gamma antibody; A kinase anchor protein 7 isoforms alpha and beta antibody; A kinase anchor protein 9 kDa antibody; A kinase anchoring protein 18 antibody; A-kinase anchor protein 18 kDa antibody; A-kinase anchor protein 7 isoforms alpha and beta antibody; AKA7A_HUMAN antibody; AKAP 18 antibody; AKAP 7 antibody; AKAP-7 isoforms alpha and beta antibody; AKAP15 antibody; AKAP18 antibody; Akap7 antibody; HGNC377 antibody; OTTHUMP00000040266 antibody; Protein kinase A anchoring protein 7 antibody; Protein kinase A anchoring protein 7 isoform alpha antibody; Protein kinase A anchoring protein 7 isoform beta antibody; Protein kinase A anchoring protein 7 isoform gamma antibody; Protein kinase A anchoring protein 7 isoforms alpha/beta antibody; Protein kinase A-anchoring protein 7 isoforms alpha/beta antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human A-kinase anchor protein 7 isoforms alpha and beta protein (1-81AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The AKAP7 Antibody (Product code: CSB-PA001529LA01HU) is Non-conjugated. For AKAP7 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA001529LB01HU	AKAP7 Antibody, HRP conjugated	ELISA
FITC	CSB-PA001529LC01HU	AKAP7 Antibody, FITC conjugated
Biotin	CSB-PA001529LD01HU	AKAP7 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC,IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium.

Gene References into Functions

AKAP7 expression levels may have clinical utility as a prognostic biomarker for post-stroke blood brain complications PMID: 28446746
Malonate in the nucleotide-binding site traps human AKAP18-gamma in a novel conformational state. PMID: 27487922
PKC tethered to AKAP7alpha was less susceptible to inhibition from the ATP-competitive inhibitor Go6976 and the substrate-competitive inhibitor PKC 20-28, but not the activation-competitive inhibitor calphostin C. PMID: 24302730
Data from studies using recombinant proteins suggest that both AKAP7-gamma and AKAP7-alpha exhibit high-affinity interactions with isoenzymes of PKC (protein kinase C); AKAP7 could dictate PKC localization/function. PMID: 22670899
AKAP7gamma is the first nuclear AKAP to bind RI and may be responsible for positioning PKA via RI and/or RII to regulate PKA-mediated gene transcription in both somatic cells and oocytes PMID: 12804576
AKAP15 regulates ENaC via a novel PKA-independent pathway PMID: 17244820
AKAP18 contains a phosphodiesterase domain that binds AMP. PMID: 18082768

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Subcellular Location

[Isoform Alpha]: Lateral cell membrane; Lipid-anchor. Note=Targeted predominantly to the lateral membrane.; [Isoform Beta]: Apical cell membrane; Lipid-anchor. Note=Targeted predominantly to the apical membrane.

Tissue Specificity

Expressed in brain, heart, lung, pancreas and skeletal muscle.

Database Links

HGNC: 377

OMIM: 604693

UniGene: Hs.486483

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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