ARHGAP29 Antibody

Datasheet

Code	CSB-PA681475LA01HU
Size	US$166
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Image	IHC image of CSB-PA681475LA01HU diluted at 1:200 and staining in paraffin-embedded mouse kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunofluorescence staining of U251 cell with CSB-PA681475LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. TheCcells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of A431 cell with CSB-PA681475LA01HU at 1:130, counter-stained with DAPI. TheCcells wereCfixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of U251 cell with CSB-PA681475LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ARHGAP29 Polyclonal antibody

Uniprot No.

Q52LW3

Target Names

ARHGAP29

Alternative Names

ARHGAP 29 antibody; Arhgap29 antibody; ARHGAP29 protein antibody; MGC166097 antibody; PARG 1 antibody; PARG1 antibody; PTPL1 associated RhoGAP 1 antibody; PTPL1-associated RhoGAP protein 1 antibody; RHG29_HUMAN antibody; Rho GTPase activating protein 29 antibody; Rho GTPase activating protein 29 isoform CRA_a antibody; Rho GTPase activating protein 29 isoform CRA_b antibody; Rho GTPase-activating protein 29 antibody; Rho-type GTPase-activating protein 29 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Rho GTPase-activating protein 29 protein (161-392AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ARHGAP29 Antibody (Product code: CSB-PA681475LA01HU) is Non-conjugated. For ARHGAP29 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA681475LB01HU	ARHGAP29 Antibody, HRP conjugated	ELISA
FITC	CSB-PA681475LC01HU	ARHGAP29 Antibody, FITC conjugated
Biotin	CSB-PA681475LD01HU	ARHGAP29 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:100-1:200
IF	1:50-1:150

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis.

Gene References into Functions

Risk haplotypes affect ARHGAP29 expression causing non-syndromic orofacial clefting. PMID: 28287101
The variant in question segregates as an autosomal dominant trait caused by an heterozygous missense variant in ARHGAP29 (p.Ser552Pro) that had not previously been identified in a population genomic databases. The p.Ser552Pro ARHGAP29 variant was not present in genomic databases and was predicted to be pathogenic by multiple in silico programs. PMID: 28029220
YAP promotes the expression of ARHGAP29 to suppress the RhoA-LIMK-cofilin pathway, destabilizing F-actin. PMID: 28538170
The of loss-of-function and ARHGAP29 missense variants in the etiology of oral clefts PMID: 27350171
Data indicate that through GTPase-activating proteins ArhGAP29 complex formation Rap1 GTP-binding protein spatially restricts Rho-mediated signaling, which is necessary for endothelial barrier potentiation. PMID: 25963656
Genetic variants in ARHGAP29 contribute to the development of nonsyndromic cleft lip with cleft palate. PMID: 25163644
We identified genetic variants in TGFB3 and ARHGAP29 associated with suboptimal healing outcome. PMID: 24635173
Rasip1-ArhGAP29 pathway also functions in Rap1-mediated regulation of endothelial junctions, which controls endothelial barrier function PMID: 23798437
ARHGAP29 is the etiologic gene for the cleft lip with or without cleft palate locus identified by genome-wide association on chromosome 1p22. PMID: 23008150
PARG1 expression was substantially reduced & it displayed at least partial promoter methylation in all investigated mantle-cell lines & in 31 primary cases. PARG1 is a strong candidate tumor suppressor gene in MCL. PMID: 17488656

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Tissue Specificity

Widely expressed. Highly expressed in skeletal muscle and heart. Expressed at intermediate level in placenta, liver and pancreas. Weakly expressed in brain, lung and kidney.

Database Links

HGNC: 30207

OMIM: 610496

KEGG: hsa:9411

STRING: 9606.ENSP00000260526

UniGene: Hs.483238

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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